| Enhancing oxygen tension and cellular function in alginate cell encapsulation devices through the use of perfluorocarbons. | |
| | |
MedLine Citation:
|
PMID: 16917927 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Encapsulation devices are often hindered by the inability to achieve sufficient oxygen levels for sustaining long-term cell survival both in vivo and in vitro. We have investigated the use of synthetic oxygen carriers in alginate gels to improve metabolic activity and viability of HepG2 cells over time. Perfluorocarbons (PFCs), specifically perfluorotributylamine (PFTBA) and perfluorooctylbromide (PFOB), were emulsified with alginate and used to encapsulate HepG2 cells in a spherical geometry. Cellular state was assessed using the MTT assay and Live/Dead stain as well as through analysis of both lactate and lactate dehydrogenase (LDH) levels which are indirect indicators of oxygen availability. Addition of 1% surfactant resulted in stable emulsions with evenly dispersed PFC droplets of the order of 1-2 microm in diameter, with no influence on cell viability. Both PFCs evaluated were effective in increasing cellular metabolic activity over alginate-only gels. The presence of 10% PFOB significantly increased cellular growth rate by 10% and reduced both intracellular LDH and extracellular lactate levels by 20-40%, improving glucose utilization efficiency. The characteristic drop in cellular metabolic activity upon encapsulation was eliminated with addition of 10% PFC and viability was better maintained throughout the bead, with a significant decrease in necrotic core size. Results were consistent under a physiologically relevant 5% oxygen environment. The incorporation of PFC synthetic oxygen carriers into encapsulation matrices has been successfully applied to improve cell function and viability with implication for a variety of tissue engineering applications. |
| | |
Authors:
|
Sarwat F Khattak; Kyuong-sik Chin; Surita R Bhatia; Susan C Roberts |
Related Documents
:
|
18068747 - Study of cell killing effect on s180 by ultrasound activating protoporphyrin ix. 18795957 - The adaptive response of anaerobically grown saccharomyces cerevisiae to hydrogen perox... 1084867 - Independence of the radiobiological oxygen constant, k, and the respiration rate of mam... 10913617 - Role of reactive oxygen species and phosphatidylinositol 3-kinase in cardiomyocyte diff... 1839887 - New techniques lead to advances in epithelial cell polarity. 21127987 - Independent and combined cytotoxicity and genotoxicity of triethylene glycol dimethacry... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Biotechnology and bioengineering Volume: 96 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2007 Jan |
Date Detail:
|
Created Date: 2006-12-05 Completed Date: 2007-02-28 Revised Date: 2007-12-03 |
Medline Journal Info:
|
Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
|
Languages: eng Pagination: 156-66 Citation Subset: IM |
Affiliation:
|
Department of Chemical Engineering, University of Massachusetts, Amherst, MA 01003, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alginates
/
chemistry* Cell Culture Techniques / methods* Cell Line Cell Proliferation Cell Survival Coated Materials, Biocompatible / chemistry Fluorocarbons / chemistry* Glucuronic Acid / chemistry Hepatocytes / cytology*, physiology* Hexuronic Acids / chemistry Humans Materials Testing Oxygen / metabolism* Tissue Engineering / methods* |
| Grant Support | |
ID/Acronym/Agency:
|
T32 GM08515/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Alginates; 0/Coated Materials, Biocompatible; 0/Fluorocarbons; 0/Hexuronic Acids; 576-37-4/Glucuronic Acid; 7782-44-7/Oxygen; 9005-32-7/alginic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of protein and carbohydrate supplementation on strength training outcome of rehabilitatio...
Next Document: Estimating haplotype relative risks in complex disease from unphased SNPs data in families using a l...