| Enhancing myocardial plasmid expression by retrograde coronary venous delivery. | |
| | |
MedLine Citation:
|
PMID: 16003788 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Myocardial delivery of genes holds great promise for treating many heart diseases; however, the optimal delivery technique, which maximizes safety and efficacy, has not been established. Two delivery techniques were evaluated in swine; percutaneous retrograde coronary venous delivery (RCVD) and direct intramyocardial injection (IM). RCVD was performed in the anterior interventricular vein (AIV) with an end-hole occlusion balloon catheter. The plasmid gWiz, encoding beta-galactosidase (10 ml; 1 mg/ml), was injected using either manual high pressure (HP-RCVD; n = 5) or pressure wire-guided low pressure (LP-RCVD; n = 4). For the IM group (n = 4), beta-Gal plasmid (5 mg/ml) was injected at 10 sites (200 microl/site) in the anterior left ventricular wall. Animals were euthanized after 5 days. The percentage of beta-Gal expressing cells in the delivered region was higher in the HP-RCVD (0.26% +/- 0.05%) than the LP-RCVD (0.05% +/- 0.03%; P = 0.07) and IM groups (0.02% +/- 0.01%; P = 0.01). Myocardium from the HP-RCVD group contained 7- and 17-fold higher levels of beta-Gal activity than either LP-RCVD and IM groups, respectively (P = 0.05 for both). The results of this study confirm the safety and efficacy of RCVD for myocardial gene delivery. |
| | |
Authors:
|
Eyas Al-Shaykh Youssef; Ping Zhang; Pamela I Rogers; Patrice Tremble; Joe Rokovich; Brian H Johnstone; Keith L March; Dongming Hou |
Related Documents
:
|
843418 - Comparison of the effects of labetalol and propranolol in healthy men at rest and durin... 14553968 - Beta-blockers in hypertension-the emperor has no clothes: an open letter to present and... 94618 - Acute and chronic beta-receptor blockage with propranolol and the cardiovascular respon... 22576288 - Relation between indices of end-organ damage and mean platelet volume in hypertensive p... 22329678 - Application of atmospheric pressure non-thermal plasma for the in vitro eradication of ... 3836858 - Changes in blood pressure and thermographic values resulting from use of a beta-blocker... |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
|
Title: Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions Volume: 65 ISSN: 1522-1946 ISO Abbreviation: Catheter Cardiovasc Interv Publication Date: 2005 Aug |
Date Detail:
|
Created Date: 2005-07-27 Completed Date: 2005-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 100884139 Medline TA: Catheter Cardiovasc Interv Country: United States |
Other Details:
|
Languages: eng Pagination: 528-34 Citation Subset: IM |
Affiliation:
|
Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Pressure / physiology Coronary Circulation / physiology* Coronary Vessels / metabolism*, physiopathology* Disease Models, Animal Female Heart Ventricles / metabolism Humans Male Models, Cardiovascular Myocardial Ischemia / metabolism, physiopathology Myocardium / metabolism*, pathology Plasmids / administration & dosage, biosynthesis* Swine beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
|
EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Drug-eluting stents for coronary bifurcations: bench testing of provisional side-branch strategies.
Next Document: Metathesis reactions in total synthesis.