Document Detail

Enhancing myocardial plasmid expression by retrograde coronary venous delivery.
MedLine Citation:
PMID:  16003788     Owner:  NLM     Status:  MEDLINE    
Myocardial delivery of genes holds great promise for treating many heart diseases; however, the optimal delivery technique, which maximizes safety and efficacy, has not been established. Two delivery techniques were evaluated in swine; percutaneous retrograde coronary venous delivery (RCVD) and direct intramyocardial injection (IM). RCVD was performed in the anterior interventricular vein (AIV) with an end-hole occlusion balloon catheter. The plasmid gWiz, encoding beta-galactosidase (10 ml; 1 mg/ml), was injected using either manual high pressure (HP-RCVD; n = 5) or pressure wire-guided low pressure (LP-RCVD; n = 4). For the IM group (n = 4), beta-Gal plasmid (5 mg/ml) was injected at 10 sites (200 microl/site) in the anterior left ventricular wall. Animals were euthanized after 5 days. The percentage of beta-Gal expressing cells in the delivered region was higher in the HP-RCVD (0.26% +/- 0.05%) than the LP-RCVD (0.05% +/- 0.03%; P = 0.07) and IM groups (0.02% +/- 0.01%; P = 0.01). Myocardium from the HP-RCVD group contained 7- and 17-fold higher levels of beta-Gal activity than either LP-RCVD and IM groups, respectively (P = 0.05 for both). The results of this study confirm the safety and efficacy of RCVD for myocardial gene delivery.
Eyas Al-Shaykh Youssef; Ping Zhang; Pamela I Rogers; Patrice Tremble; Joe Rokovich; Brian H Johnstone; Keith L March; Dongming Hou
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions     Volume:  65     ISSN:  1522-1946     ISO Abbreviation:  Catheter Cardiovasc Interv     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-07-27     Completed Date:  2005-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100884139     Medline TA:  Catheter Cardiovasc Interv     Country:  United States    
Other Details:
Languages:  eng     Pagination:  528-34     Citation Subset:  IM    
Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
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MeSH Terms
Blood Pressure / physiology
Coronary Circulation / physiology*
Coronary Vessels / metabolism*,  physiopathology*
Disease Models, Animal
Heart Ventricles / metabolism
Models, Cardiovascular
Myocardial Ischemia / metabolism,  physiopathology
Myocardium / metabolism*,  pathology
Plasmids / administration & dosage,  biosynthesis*
beta-Galactosidase / metabolism
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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