Document Detail


Enhancing the lethal effect of high-intensity pulsed electric field in milk by antimicrobial compounds as combined hurdles.
MedLine Citation:
PMID:  18420606     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
High-intensity pulsed electric field (HIPEF) is a nonthermal treatment studied for its wide antimicrobial spectrum on liquid food, including milk and dairy products. Moreover, the antimicrobial effect of HIPEF may be enhanced by combining HIPEF with other treatments as hurdles. Nisin and lysozyme are natural antimicrobial compounds that could be used in combination with HIPEF. Therefore, the purpose of this study was to determine the effect of combining HIPEF with the addition of nisin and lysozyme to milk inoculated with Staphylococcus aureus with regard to different process variables. The individual addition of nisin and lysozyme did not produce any reduction in cell population within the proposed range of concentrations, whereas their combination resulted in a pH-dependent microbial death of Staph. aureus. The addition of nisin and lysozyme to milk combined with HIPEF treatment resulted in a synergistic effect. Applying a 1,200-micros HIPEF treatment time to milk at pH 6.8 containing 1 IU/mL of nisin and 300 IU/mL of lysozyme resulted in a reduction of more than 6.2 log units of Staph. aureus. Final counts resulting from the addition of nisin and lysozyme and applying HIPEF strongly depended on both the sequence of application and the milk pH. Thus, more research is needed to elucidate the mode of action of synergism as well as the role of different process variables, although the use of HIPEF in combination with antimicrobial compounds such as nisin and lysozyme is shown to be potentially useful in processing milk and dairy products.
Authors:
A Sobrino-López; O Martín-Belloso
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of dairy science     Volume:  91     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-18     Completed Date:  2008-06-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1759-68     Citation Subset:  IM    
Affiliation:
Department of Food Technology, University of Lleida, 25198-Lleida, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Infective Agents / pharmacology*
Electricity
Food Handling / methods*
Food Preservation / methods*
Hydrogen-Ion Concentration
Milk / microbiology*
Muramidase / pharmacology
Nisin / pharmacology
Staphylococcus aureus / drug effects
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 1414-45-5/Nisin; EC 3.2.1.17/Muramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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