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Enhancing enzymatic saccharification of waste newsprint by surfactant addition
MedLine Citation:
PMID:  9694689     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Several nonionic surfactants, Pluronic F68 and F88 (BASF) as well as Tween 20 and 80, were found in this study to enhance the enzymatic hydrolysis of pretreated newsprint. Pluronic F68 was the most effective among the surfactants studied. With 2% (w/v) F68 the cellulose (10 g/L) conversion achieved with 2 g/L cellulase reached 52%, about the same as the 48% conversion achieved with 10 g/L cellulase in a surfactant-free system. An empirical model, x = Kt0. 5-(t/tau)1/2, has been developed to describe the extended hydrolysis, where x is conversion, t is reaction time, and K and tau correspond to the initial saccharification rate and the characteristic time for its tendency to deviate from the initial power-law (exponent = 0.5) behavior. The best-fit K and tau in the extended hydrolysis experiments confirmed that F68 significantly enhanced both the initial rate and long-term hydrolysis. A study was also made to evaluate the compatibility of these surfactants, at concentrations up to 2% (w/v), with cultures of Saccharomyces cerevisiae and Dekkera clausenii. While Tween 20 was found strongly inhibitory to D. clausenii, the culture growth in all other surfactant-added systems was comparable to that in the surfactant-free controls.
Authors:
Wu; Ju
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Biotechnology progress     Volume:  14     ISSN:  1520-6033     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-08-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  -    
Other Details:
Languages:  Eng     Pagination:  649-52     Citation Subset:  -    
Affiliation:
Department of Chemical Engineering, The University of Akron, Akron, Ohio 44325-3906, USA.
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