| Enhancing the 3-hydroxyvalerate component in bioplastic PHBV production by Cupriavidus necator. | |
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MedLine Citation:
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PMID: 21905226 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In the current context of global warming, the substitution of conventional plastics with bioplastics is a challenge. To take it up we must meet technical and financial requirements. In the case of PHBV, technical properties' modulation can be acquired via 3-hydroxyvalerate content modification. Yet 3-hydroxyvalerate enhancement is an issue; simultaneous evaluation of several 3-hydroxyvalerate-enhancing substrates through fractional factorial design of experiments is described. Eight substrates were studied: citric, valeric, propionic and levulinic acids, propanol, pentanol and sodium propionate - for 3-hydroxyvalerate enhancement, and sodium glutamate - for biomass and PHA enhancement. The most efficient 3-hydroxyvalerate enhancing factors were found to be levulinic acid, sodium propionate and pentanol. However, pentanol, at 1 g/L concentration, also showed extremely negative influence on biomass production and on the PHA content in cells. The effect of the inoculum nutrient composition on the final 3-hydroxyvalerate content was also evaluated. These results showed that the most efficient combination for the production of high 3-hydroxyvalerate content PHBV was primary inoculum growth on mineral medium followed by 48 hours fermentation with levulinic acid and sodium propionate, at 1 g/L both, as only carbon sources. This allowed us the production of PHBV with 80 mol % 3-hydroxyvalerate content, overall volumetric and specific productivities of 2 mg/L/h and 3.9 mg/g(CDW) /h respectively with only 2 g/L of inducing substances addition. |
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Authors:
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Nathalie Berezina |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-9 |
Journal Detail:
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Title: Biotechnology journal Volume: - ISSN: 1860-7314 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101265833 Medline TA: Biotechnol J Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Materia Nova, rue des Foudriers 1, 7822 Ghislenghien, Belgium. nathalie.berezina@materianova.be. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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