Document Detail


Enhancement of tissue engineered bone formation by a low pressure system improving cell seeding and medium perfusion into a porous scaffold.
MedLine Citation:
PMID:  16552848     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To obtain more extensive bone formation in composites of porous ceramics and bone marrow stromal cells (BMSCs), we hypothesized that a low-pressure system would serve to facilitate the perfusion of larger number of BMSCs into the porous scaffold, enhancing bone formation within the composites. After culturing BMSCs in osteogenic medium, porous blocks of beta-tricalcium phosphate (beta-TCP) were soaked in the cell suspension. Composites of the block and BMSCs were put immediately into a vacuum desiccator. Low pressure was applied to the low pressure group, while controls were left at atmospheric pressure. Composites were incubated in vitro or subcutaneously implanted into syngeneic rats, then analyzed biologically and histologically. In the in vitro group, cell suspension volume, cell seeding efficiency, alkaline phosphatase (ALP) activity, and DNA content in the beta-TCP blocks were significantly higher in low pressure group than in the controls. Scanning electron microscopy (SEM) demonstrated that a greater number of cells covered the central parts of the composites in the low pressure group. ALP activity in the composites was increased at 3 and 6 weeks after implantation into rats. Histomorphometric analysis revealed more uniform and extensive bone formation in the low pressure group than in the controls. The application of low pressure during the seeding of BMSCs in perfusing medium into a porous scaffold is useful for tissue-engineered bone formation.
Authors:
Juyong Wang; Yoshinori Asou; Ichiro Sekiya; Shinichi Sotome; Hisaya Orii; Kenichi Shinomiya
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Biomaterials     Volume:  27     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-03-21     Completed Date:  2006-04-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  2738-46     Citation Subset:  IM    
Affiliation:
Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, 113-8519, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biocompatible Materials / chemistry
Calcium Phosphates / chemistry*
Cell Culture Techniques / methods*
Cell Differentiation
Cell Proliferation
Cell Survival
Cells, Cultured
Hematopoietic Stem Cells / cytology*,  physiology
Male
Materials Testing
Osteoblasts / cytology*,  physiology
Osteogenesis / physiology*
Perfusion / methods
Porosity
Pressure
Rats
Rats, Inbred F344
Tissue Engineering / methods
Vacuum
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Calcium Phosphates; 0/beta-tricalcium phosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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