Document Detail

Enhancement of sphingosine 1-phosphate-induced phospholipase C activation during G(0)-G(1) transition in rat hepatocytes.
MedLine Citation:
PMID:  15215654     Owner:  NLM     Status:  MEDLINE    
We previously reported that sphingosine 1-phosphate (S1P) induces inhibition of adenylyl cyclase and activation of phospholipase C via independent G protein-coupled receptors in adult rat hepatocytes. Although S1P activation of phospholipase C and subsequent increase of intracellular Ca(2+) concentration were enhanced during the primary culture of hepatocytes, S1P inhibition of adenylyl cyclase remained unchanged. Here, we addressed whether enhancement of S1P-induced actions is dependent on change of status from the differentiated (G(0)) phase to proliferating (G(1)/S) phase in hepatocytes. By employing cell-density-dependency of the transition (G(0)-G(1)) of hepatocytes in primary culture in vitro, it was found that the enhancement of phospholipase C activation by S1P was dependent on cell density and correlated to the G(0)-G(1) transition. The correlation was further confirmed in vivo by 70% hepatectomy as a proliferating hepatocytes model. Northern blot analysis suggested an enhanced expression of S1P(2) receptor in proliferating hepatocytes.
Dong-Soon Im; Hideaki Tomura; Masayuki Tobo; Masayuki Tobe; Koichi Sato; Fumikazu Okajima
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacological sciences     Volume:  95     ISSN:  1347-8613     ISO Abbreviation:  J. Pharmacol. Sci.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-24     Completed Date:  2005-01-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  101167001     Medline TA:  J Pharmacol Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  284-90     Citation Subset:  IM    
Laboratory of Pharmacology, College of Pharmacy, Pusan National University, Pusan, Republic of Korea.
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MeSH Terms
Blotting, Northern
Cell Division / drug effects
Cell Separation
DNA / biosynthesis
Enzyme Activators / pharmacology*
G0 Phase / drug effects*
G1 Phase / drug effects*
Hepatocytes / drug effects,  metabolism*
Inositol Phosphates / biosynthesis
Liver Regeneration / drug effects
Lysophospholipids / pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Sphingosine / analogs & derivatives*,  pharmacology*
Tissue Culture Techniques
Type C Phospholipases / metabolism*
Up-Regulation / drug effects
Reg. No./Substance:
0/Enzyme Activators; 0/Inositol Phosphates; 0/Lysophospholipids; 123-78-4/Sphingosine; 26993-30-6/sphingosine 1-phosphate; 9007-49-2/DNA; EC 3.1.4.-/Type C Phospholipases
Erratum In:
J Pharmacol Sci. 2004 Jul;95(3):402
Note: Tobe, Masayuki [corrected to Tobo, Masayuki]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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