Document Detail

Enhancement of silicon using micro-patterned surfaces of thin films.
MedLine Citation:
PMID:  20379964     Owner:  NLM     Status:  MEDLINE    
Micro-textured biomaterials might enhance cytocompatibility of silicon-based micro-electro-mechanical system (bio-MEMS) dummies. Photolithography-physical vapour deposition was used to produce diamond-like carbon (DLC) or Ti squares and circles on silicon, and also their inverse replicas; then DLC and Ti were compared for their guiding potential, using a SaOS-2 cell model. Scanning electron microscopy at 48 hours indicated cells were well-spread on large-sized patterns (several cells on one pattern) and assumed the geometrical architecture of underlying features. Medium-sized patterns (slightly smaller than solitary indicator cells) were inhabited by singular cells, which stretched from one island to another, assuming longitudinal or branching morphologies. On small-sized patterns (much smaller than individual cells;rpar; cells covered large micro-textured areas, but cellular filopodia bypassed the bare silicon. Immunofluorescence and confocal laser scanning microscopy indicated that the actin cytoskeleton and vinculin-containing adhesion junctions were present on the patterned areas, but not on the bare silicon. Cell density/coverage disclosed a 3.4-3.7-fold preference for the biomaterial patterns over silicon substrate (p 0.001). Differences in the cellular response between materials were lost at 120 hours when cells were confluent. The working hypothesis was proven; enhancement by micro-patterning depends on the pattern size, shape and material and can be used to improve biocompatibility during the initial integration phase of the device.
Emilia Kaivosoja; Sami Myllymaa; Vesa-Petteri Kouri; Katja Myllymaa; Reijo Lappalainen; Yrj? T Konttinen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-09
Journal Detail:
Title:  European cells & materials     Volume:  19     ISSN:  1473-2262     ISO Abbreviation:  Eur Cell Mater     Publication Date:  2010  
Date Detail:
Created Date:  2010-04-09     Completed Date:  2010-06-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973416     Medline TA:  Eur Cell Mater     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  147-57     Citation Subset:  IM    
Department of Medicine, Institute of Clinical Medicine, Helsinki University Central Hospital, Haartmaninkatu 8, FI-00029 HUS, Finland.
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MeSH Terms
Biocompatible Materials / chemistry*
Cell Adhesion / physiology
Cell Line, Tumor
Cell Shape / physiology
Coated Materials, Biocompatible
Cytoskeleton / physiology,  ultrastructure
Materials Testing / methods*
Membranes, Artificial*
Micro-Electrical-Mechanical Systems / methods*,  standards
Microscopy, Electron, Scanning
Pseudopodia / physiology
Silicon / chemistry*
Surface Properties
Reg. No./Substance:
0/Biocompatible Materials; 0/Coated Materials, Biocompatible; 0/Membranes, Artificial; 7440-21-3/Silicon

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