Document Detail

Enhancement of recombinant human endostatin on the radiosensitivity of human pulmonary adenocarcinoma A549 cells and its mechanism.
MedLine Citation:
PMID:  22778546     Owner:  NLM     Status:  MEDLINE    
We observed the effects of endostar on the radiosensitivity of pulmonary adenocarcinoma A549 cells and found that endostar inhibited A549 cell growth under normoxia and hypoxia in time and dose-dependent manners; the D(0) and D(q) values in control and endostar groups were (1.36 and 1.30) versus (1.019 and 1.015) under normoxia and (1.693 and 1.39) versus (2.453 and 1.026) under hypoxia, respectively; SER was 1.04 under normoxia and 1.22 under hypoxia in endostar group; under normoxia, the apoptosis rates in control, radiotherapy, endostar and combination groups were 15.9 ± 0.57%, 42.7 ± 0.37%, 19.9 ± 0.48%, and 41.5 ± 0.38%, respectively, with no significant difference between combination and radiotherapy groups; there was significant difference in G(2)/M phase cells between combination and radiotherapy groups (P = 0.028); under hypoxia, the apoptosis rates in the four groups were 16.7 ± 0.67%, 30.1 ± 0.95%, 26.7 ± 0.62%, and 36.3 ± 0.71%, respectively, with significant difference between combination and radiotherapy groups; G(2)/M phase cells were higher in combination group than radiotherapy group (P = 0.000); G(2)/M phase cells were higher in hypoxic combination group than in normoxic combination group (P = 0.003). Based on these results, we conclude that under hypoxia, endostar can enhance the radiosensitivity of A549 cells through G(2)/M arrest.
Xiao-dong Jiang; Yun Qiao; Peng Dai; Qin Chen; Jin Wu; Da-an Song; Shi-qiu Li
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-18
Journal Detail:
Title:  Journal of biomedicine & biotechnology     Volume:  2012     ISSN:  1110-7251     ISO Abbreviation:  J. Biomed. Biotechnol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-07-10     Completed Date:  2013-01-07     Revised Date:  2013-07-12    
Medline Journal Info:
Nlm Unique ID:  101135740     Medline TA:  J Biomed Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  301931     Citation Subset:  IM    
Department of Radiology, Lianyungang First People's Hospital, Jiangsu, Lianyungang 222002, China.
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MeSH Terms
Adenocarcinoma / drug therapy*,  metabolism,  pathology,  radiotherapy*
Analysis of Variance
Apoptosis / drug effects,  radiation effects
Cell Cycle
Cell Hypoxia / physiology
Cell Line, Tumor
Cell Survival / drug effects,  radiation effects
Endostatins / pharmacology*
Lung Neoplasms / drug therapy*,  metabolism,  pathology,  radiotherapy*
Radiation Tolerance / drug effects*
Radiation-Sensitizing Agents / pharmacology*
Recombinant Proteins / pharmacology
Reg. No./Substance:
0/Endostatins; 0/Radiation-Sensitizing Agents; 0/Recombinant Proteins; 0/endostar protein

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