Document Detail

Enhancement of proteolytic enzyme activity excreted from Bacillus stearothermophilus for a thermophilic aerobic digestion process.
MedLine Citation:
PMID:  12003317     Owner:  NLM     Status:  MEDLINE    
Proteolysis is one of the main enzymatic reactions involved in waste activated sludge (WAS) digestion. In this study, proteases excreted from Bacillus stearothermophilus (ATCC 31197) were classified, and an enhancement of protease activity was achieved using economical chemical additives for WAS digestion. Proteases excreted from B. stearothermophilus were classified into two families: serine and metallo-proteases. Various metal ions were investigated as additives which could potentially enhance protease activity. It was observed that Ca2+ and Fe2+ could markedly activate these enzymes. These results were applied to thermophilic aerobic digestion (TAD) of industrial WAS using B. stearothermophilus. The addition of these divalent ions enhanced the degradation performance of the TAD process in terms of reducing the total suspended solids (TSSs), the dissolved organic carbon (DOC) content, and the intracellular and extracellular protein concentrations. The best result, with respect to protein reduction in a digestion experiment, was obtained by the addition of 2 mM Ca2+. Therefore, a proposed TAD process activated by calcium addition can be successfully used for industrial and municipal WAS digestion to the upgrading of TAD process performance.
Young-Kee Kim; Jin-Hye Bae; Byung-Keun Oh; Won Hong Lee; Jeong-Woo Choi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioresource technology     Volume:  82     ISSN:  0960-8524     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-10     Completed Date:  2002-12-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  157-64     Citation Subset:  IM    
Department of Chemical Engineering, Sogang Unirersity, Seoul, South Korea.
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MeSH Terms
Calcium / chemistry,  pharmacology
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Geobacillus stearothermophilus / enzymology*
Hydrogen-Ion Concentration
Iron / pharmacology
Metalloendopeptidases / chemistry
Models, Statistical
Serine Endopeptidases / chemistry
Time Factors
Zinc / pharmacology
Reg. No./Substance:
0/Cations; 0/Ions; 7439-89-6/Iron; 7440-66-6/Zinc; 7440-70-2/Calcium; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.24.-/Metalloendopeptidases
Erratum In:
Bioresour Technol 2002 Jul;83(3):271

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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