| Enhancement of the predicted drug hepatotoxicity in gel entrapped hepatocytes within polysulfone-g-poly (ethylene glycol) modified hollow fiber. | |
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MedLine Citation:
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PMID: 20816885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Collagen gel-based 3D cultures of hepatocytes have been proposed for evaluation of drug hepatotoxicity because of their more reliability than traditional monolayer culture. The collagen gel entrapment of hepatocytes in hollow fibers has been proven to well reflect the drug hepatotoxicity in vivo but was limited by adsorption of hydrophobic drugs onto hollow fibers. This study aimed to investigate the impact of hollow fibers on hepatocyte performance and drug hepatotoxicity. Polysulfone-g-poly (ethylene glycol) (PSf-g-PEG) hollow fiber was fabricated and applied for the first time to suppress the drug adsorption. Then, the impact of hollow fibers was evaluated by detecting the hepatotoxicity of eight selected drugs to gel entrapped hepatocytes within PSf and PSf-g-PEG hollow fibers, or without hollow fibers. The hepatocytes in PSf-g-PEG hollow fiber showed the highest sensitivity to drug hepatotoxicity, while those in PSf hollow fiber and cylindrical gel without hollow fiber underestimated the hepatotoxicity due to either drug adsorption or low hepatic functions. Therefore, the 3D culture of gel entrapped hepatocytes within PSf-g-PEG hollow fiber would be a promising tool for investigation of drug hepatotoxicity in vitro. |
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Authors:
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Chong Shen; Guoliang Zhang; Qin Meng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-15 |
Journal Detail:
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Title: Toxicology and applied pharmacology Volume: 249 ISSN: 1096-0333 ISO Abbreviation: Toxicol. Appl. Pharmacol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-26 Completed Date: 2010-11-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0416575 Medline TA: Toxicol Appl Pharmacol Country: United States |
Other Details:
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Languages: eng Pagination: 140-7 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals Cells, Cultured Collagen Cytochrome P-450 Enzyme System / metabolism Drug Toxicity Gels Hepatocytes / cytology, drug effects*, enzymology Male Membranes, Artificial Polyethylene Glycols* Polymers* Rats Rats, Sprague-Dawley Sulfones* Toxicity Tests / methods* |
| Chemical | |
Reg. No./Substance:
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0/Gels; 0/Membranes, Artificial; 0/Polyethylene Glycols; 0/Polymers; 0/Sulfones; 25135-51-7/polysulfone P 1700; 9007-34-5/Collagen; 9035-51-2/Cytochrome P-450 Enzyme System |
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