Document Detail


Enhancement of polymorphonuclear cell phagocytosis by lipid A-activated monocytes via cell-to-cell contact: a possible role for membrane-associated interleukin-6 and interleukin-8.
MedLine Citation:
PMID:  7759774     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polymorphs (PMN) and monocytes/macrophages (Mo) play a very important role in the host defence since they participate to inflammatory processes, tissue repairing and antitumor activity. Previous studies showed that lipopolysaccharide (LPS)-activated Mo are able to upregulate PMN phagocytic ability via cell-to-cell contact mechanisms mediated by bound to Mo membrane (m) cytokines (CKs), such as Tumor Necrosis Factor (TNF)-alpha, Interleukin (IL)-1 alpha and IL-1 beta. Based on these grounds, the role of Mo m-associated IL-6 and IL-8 on the modulation of PMN activity has been evaluated. In the first step, PMN incubated with lipid A (LA)-activated Mo showed an increased phagocytosis dependent on cell-to-cell contact only. In the second step, LA-activated Mo were pretreated with antirecombinant human (Rhu) IL-6 and IL-8 monoclonal antibodies (MoAbs), respectively and, in such a way, the enhanced phagocytic activity of PMN was abrogated. In the third step, PMN incubated with LA-activated supernatants (AS) from PBMC cultures exhibited an enhanced phagocytic activity, that was abrogated when LA-AS were pretreated with anti-Rhu IL-6 and anti-Rhu IL-8 MoAbs, respectively. These data suggest that IL-6 and IL-8 associated to Mo membrane may modulate PMN activation through a cell-to-cell contact dependent pathway.
Authors:
P Decandia; M Serrone; L Pestillo; M R Ribaud; C De Simone; C Tortorella; S Antonaci; E Jirillo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunopharmacology and immunotoxicology     Volume:  17     ISSN:  0892-3973     ISO Abbreviation:  Immunopharmacol Immunotoxicol     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-06-29     Completed Date:  1995-06-29     Revised Date:  2009-05-14    
Medline Journal Info:
Nlm Unique ID:  8800150     Medline TA:  Immunopharmacol Immunotoxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  49-58     Citation Subset:  IM    
Affiliation:
University of Bari Medical Faculty, Policlinico, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal / immunology
Binding, Competitive / immunology
Cell Adhesion / immunology*
Humans
Interleukin-6 / immunology
Interleukin-8 / immunology
Lipid A / immunology
Macrophages / immunology*
Neutrophils / immunology*
Phagocytosis / immunology*
Recombinant Proteins / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Interleukin-6; 0/Interleukin-8; 0/Lipid A; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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