Document Detail

Enhancement of phorbol ester induced cell aggregation after alterations in asparagine-linked oligosaccharides.
MedLine Citation:
PMID:  8401289     Owner:  NLM     Status:  MEDLINE    
Human erythroleukemia (K-562) cells grown in the presence of phorbol 12,13-dibutyrate formed aggregates of cells not seen in untreated control cultures. Furthermore, the proportion of cells in aggregates and the size of the aggregates both increased dramatically in cultures treated with both phorbol ester and kifunensine, an inhibitor of asparagine-linked oligosaccharide processing. Relative to control cells, phorbol ester treated cells exhibited a greater proportion of N-linked oligosaccharides of the complex-type. Kifunensine prevented this change and caused an accumulation of Man9GlcNAc2. The enhanced aggregation of cells treated with phorbol ester plus kifunensine depended on phorbol ester concentration and was blocked by inhibitors of protein kinase C (H7, sphinganine and sangivamycin). In flow cytometry analysis, phorbol ester treated K-562 cells showed an increase in CD44, a glycoprotein involved in cell adhesion. Moreover, monoclonal antibody to CD44 augmented reaggregation of phorbol ester treated cells. The results implicate phorbol ester induction of CD44 in aggregation of K-562 cells and demonstrate that the presence of high mannose-type asparagine-linked oligosaccharides on cell glycoproteins correlates with increased aggregation of phorbol ester treated cells.
P B Ahrens
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of the cell / under the auspices of the European Cell Biology Organization     Volume:  77     ISSN:  0248-4900     ISO Abbreviation:  Biol. Cell     Publication Date:  1993  
Date Detail:
Created Date:  1993-10-29     Completed Date:  1993-10-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8108529     Medline TA:  Biol Cell     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  255-60     Citation Subset:  IM    
Department of Biochemistry, Medical College of Wisconsin, Milwaukee 53226.
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MeSH Terms
Alkaloids / pharmacology*
Asparagine / chemistry*
Carbohydrate Conformation
Cell Aggregation / drug effects
Drug Synergism
Oligosaccharides / chemistry*
Phorbol 12,13-Dibutyrate / pharmacology*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Alkaloids; 0/Oligosaccharides; 109944-15-2/kifunensine; 37558-16-0/Phorbol 12,13-Dibutyrate; 7006-34-0/Asparagine

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