Document Detail


Enhancement of phagocytic and chemokinetic activities of rainbow trout head kidney cells by C-reactive protein.
MedLine Citation:
PMID:  10048559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate immunostimulating activity of purified rainbow trout (Oncorhynchus mykiss) C-reactive protein (CRP) on trout phagocytic cells. ANIMALS: 20 rainbow trout and 2 rabbits. PROCEDURE: The effect of CRP on phagocytic activity of head kidney (HK) cells was examined by use of a phagocytosis assay with plastic particles. The enhancing effect of CRP on migration activity of HK cells was examined by use of the blind well assay. RESULTS: Glass-adherent cells from clinically normal trout had increased dose-dependent phagocytic activity against plastic particles when cells were incubated in the presence of CRP. Pretreatment of particles with CRP also enhanced phagocytic activity of the cells, indicating an opsonic effect of CRP. Rabbit anti-trout CRP serum suppressed the enhancing activity of CRP. The HK cells had significant dose-dependent chemokinetic activity against CRP that was not inhibited by anti-CRP serum, indicating that a CRP-antibody complex also could be chemokinetic. CONCLUSIONS: Rainbow trout CRP has immunostimulating activity for HK cells, resulting in enhanced phagocytic and chemokinetic activities.
Authors:
H Kodama; H Arimitsu; M Mukamoto; C Sugimoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of veterinary research     Volume:  60     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-06     Completed Date:  1999-05-06     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  240-4     Citation Subset:  IM    
Affiliation:
Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
C-Reactive Protein / immunology*,  isolation & purification
Cell Movement / immunology,  physiology
Cells, Cultured
Chromatography, Ion Exchange / veterinary
Electrophoresis, Polyacrylamide Gel / veterinary
Immune Sera / biosynthesis
Immunodiffusion / veterinary
Kidney / cytology
Microspheres
Oncorhynchus mykiss / immunology*
Opsonin Proteins / chemistry,  immunology
Phagocytes / physiology*
Phagocytosis / physiology*
Rabbits
Sequence Analysis
Chemical
Reg. No./Substance:
0/Immune Sera; 0/Opsonin Proteins; 9007-41-4/C-Reactive Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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