Document Detail


Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells.
MedLine Citation:
PMID:  15515346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The use of suitable cells transplanted into periodontal osseous defects appears to be a powerful strategy to promote periodontal tissue regeneration. Mesenchymal stem cells (MSCs) isolated from bone marrow have the potential for multilineage differentiation. The purpose of this study was to examine whether auto-transplantation of MSCs into periodontal osseous defects would be useful for periodontal tissue regeneration. METHODS: Bone marrow MSCs were isolated from beagle dogs and expanded in vitro. The expanded MSCs were mixed with atelocollagen (2% type I collagen) at final concentrations of 2 x 10(6), 5 x 10(6), 1 x 10(7), or 2 x 10(7) cells/ml, and auto-transplanted into experimental Class III defects. Atelocollagen alone was implanted into the defects as a control. Periodontal tissue healing was evaluated by histological and morphometric analyses 1 month after transplantation. RESULTS: The defects were regenerated with cementum, periodontal ligament, and alveolar bone in the MSC-atelocollagen groups. Less periodontal tissue regeneration was observed in the control group compared to the MSC-atelocollagen groups. Morphometric analysis revealed that the percentage of new cementum length in the 5 x 10(6) and 2 x 10(7) cells/ml groups and the percentage of new bone area in the 2 x 10(7) cells/ml group were significantly higher than in the control group (P < 0.01). CONCLUSION: These findings suggest that auto-transplantation of bone marrow mesenchymal stem cells is a novel option for periodontal tissue regeneration.
Authors:
Hiroyuki Kawaguchi; Akio Hirachi; Naohiko Hasegawa; Tomoyuki Iwata; Hidenori Hamaguchi; Hideki Shiba; Takashi Takata; Yukio Kato; Hidemi Kurihara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of periodontology     Volume:  75     ISSN:  0022-3492     ISO Abbreviation:  J. Periodontol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-11-01     Completed Date:  2004-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1281-7     Citation Subset:  D; IM    
Affiliation:
Department of Periodontal Medicine, Division of Frontier Medical Science, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan. hkawa@hiroshima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Alveolar Bone Loss / pathology,  surgery*
Alveolar Process / pathology
Animals
Bone Marrow Transplantation*
Bone Regeneration / physiology*
Cell Differentiation
Cell Lineage
Collagen
Dental Cementum / pathology
Dogs
Mesenchymal Stem Cell Transplantation*
Periodontal Ligament / pathology
Transplantation, Autologous
Wound Healing / physiology
Chemical
Reg. No./Substance:
0/atelocollagen; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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