Document Detail


Enhancement of neutral lipid productivity in the microalga Isochrysis affinis galbana (T-Iso) by a mutation-selection procedure.
MedLine Citation:
PMID:  22614222     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Microalgae offer a high potential for energetic lipid storage as well as high growth rates. They are therefore considered promising candidates for biofuel production, with the selection of high lipid-producing strains a major objective in projects on the development of this technology. We developed a mutation-selection method aimed at increasing microalgae neutral lipid productivity. A two step method, based on UVc irradiation followed by flow cytometry selection, was applied to a set of strains that had an initial high lipid content and improvement was assessed by means of Nile-red fluorescence measurements. The method was first tested on Isochrysis affinis galbana (T-Iso). Following a first round of mutation-selection, the total fatty acid content had not increased significantly, being $262\pm 21\;{\rm mgTFA}\cdot {\rm (gC)}^{- {\rm 1}} $ for the wild type (WT) and $269\pm 49\;{\rm mgTFA}\cdot {\rm (gC)}^{- {\rm 1}} $ for the selected population (S1M1). Conversely, fatty acid distribution among the lipid classes was affected by the process, resulting in a 20% increase for the fatty acids in the neutral lipids and a 40% decrease in the phospholipids. After a second mutation-selection step (S2M2), the total fatty acid content reached $409\pm 64\;{\rm mgTFA}\cdot {\rm (gC)}^{- {\rm 1}} $ with a fatty acid distribution similar to the S1M1 population. Growth rate remained unaffected by the process, resulting in a 80% increase for neutral lipid productivity. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.
Authors:
Gaël Bougaran; Catherine Rouxel; Nolwenn Dubois; Raymond Kaas; Sophie Grouas; Ewa Lukomska; Jean-René Le Coz; Jean-Paul Cadoret
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-21
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  -     ISSN:  1097-0290     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
IFREMER-PBA, rue de l'Ile d'Yeu, BP 21105 - 44311 Nantes cedex 03, France. gael.bougaran@ifremer.fr.
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