Document Detail

Enhancement of lung-colonizing ability of cloned low-metastatic Lewis lung carcinoma cells by treatment with highly polar compounds.
MedLine Citation:
PMID:  6540247     Owner:  NLM     Status:  MEDLINE    
Treatment of low-metastatic Lewis lung carcinoma cells (P-29) with dimethylsulfoxide (DMSO) in vitro enhanced their lung-colonizing ability. The effects of other highly polar compounds on the lung-colonizing ability of P-29 cells were examined. The following compounds were found to enhance the lung-colonizing ability of the cells: acetamide, N-methylacetamide, N-methylformamide, N,N-dimethylformamide, piperidone and hexamethylphosphoric triamide. Treatment of P-29 cells with DMSO or other polar compounds resulted in increases in activities of degradative enzymes, such as cathepsin B and plasminogen activator. The increases in cathepsin B and plasminogen activator activities were apparent after a 24 h treatment with DMSO and were suppressed by simultaneous treatment with cycloheximide, which suggested that they were due to syntheses of new proteins. DMSO-treated P-29 cells degraded [3H]leucine-labelled subendothelial matrix much more than did untreated cells. P-29 cells treated with DMSO or other polar compounds became attached to culture dishes more rapidly and were more resistant to detachment by trypsin treatment than untreated cells. A significant correlation was found between the degrees of adhesiveness of P-29 cells treated with various polar compounds and their lung-colonizing abilities.
K Takenaga
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  34     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-09-04     Completed Date:  1984-09-04     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  83-9     Citation Subset:  IM    
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MeSH Terms
Carcinoma / pathology*
Cathepsins / metabolism
Cell Adhesion / drug effects
Cycloheximide / pharmacology
Dimethyl Sulfoxide / pharmacology
Lung Neoplasms / secondary*
Mice, Inbred C57BL
Neoplasm Metastasis*
Plasminogen Activators / metabolism
Structure-Activity Relationship
Trypsin / diagnostic use
Reg. No./Substance:
66-81-9/Cycloheximide; 67-68-5/Dimethyl Sulfoxide; EC 3.4.-/Cathepsins; EC 3.4.21.-/Plasminogen Activators; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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