| Enhancement of iron-catalyzed free radical formation by acidosis in brain homogenates: differences in effect by lactic acid and CO2. | |
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MedLine Citation:
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PMID: 2492027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of lactic acidosis and of extreme hypercapnia on free radical generation and lipid peroxidation in brain tissues was studied. Cortical homogenates were prepared from the rat brain in a bicarbonate buffer and incubated for 60 min. Lipid peroxidation was evaluated by measurements of thiobarbituric acid reactive (TBAR) material and alpha-tocopherol analysis. The pH during incubations were decreased to 6.10-6.20 by either lactic acid administration or equilibration with 60% CO2 gas in paired experiments. In homogenates treated with lactic acid there was a 20-fold increase in TBAR material and the alpha-tocopherol concentration decreased to approximately 60% of control. There was only a 10-fold increase in TBAR material and no change in alpha-tocopherol concentration if acidosis was induced by CO2. These differences between lactic acidosis and hypercapnic acidosis were statistically highly significant. The results indicate that lactic acidosis has a more pronounced effect in augmenting free radical generation in brain tissues than acidosis due to an increase in CO2 tension. It is suggested that this effect of lactic acid is mediated by increased dissociation of catalytic iron from proteins of the transferrin type. |
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Authors:
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S Rehncrona; H N Hauge; B K Siesjö |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism Volume: 9 ISSN: 0271-678X ISO Abbreviation: J. Cereb. Blood Flow Metab. Publication Date: 1989 Feb |
Date Detail:
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Created Date: 1989-02-17 Completed Date: 1989-02-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8112566 Medline TA: J Cereb Blood Flow Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 65-70 Citation Subset: IM |
Affiliation:
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Laboratory of Experimental Brain Research, University of Lund, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acidosis, Lactic
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metabolism* Animals Bicarbonates / metabolism Brain / metabolism* Carbon Dioxide / metabolism* Free Radicals Iron / metabolism Lipid Peroxides / metabolism Male Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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5 RO1 NS-07838/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Bicarbonates; 0/Free Radicals; 0/Lipid Peroxides; 124-38-9/Carbon Dioxide; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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