| Enhancement of the innate and cellular immune response in patients with genital warts treated with topical imiquimod cream 5%. | |
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MedLine Citation:
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PMID: 10480263 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism of action of imiquimod 5% cream applied topically to patients with genital warts was evaluated in a double-blind, placebo-controlled study. Imiquimod (16 patients) or placebo (three patients) was applied three times per week for up to 16 weeks. All imiquimod-treated patients had a > or =75% reduction in total wart area while only one of three placebo-treated patients had a similar reduction. Wart biopsies were taken at prestudy, week 6, and end of treatment. Polymerase chain reaction (PCR) for human papillomavirus (HPV) DNA and reverse transcriptase (RT)-PCR for messenger (m)RNAs were used to identify cytokines, cellular markers, viral gene products, and cell cycle markers in these biopsies. Treatment with imiquimod, an immune response modifier, stimulated significant increases in mRNA for interferon (IFN)-alpha, IFN-gamma and 2',5' oligoadenylate synthetase (2',5'-AS) as well as a tendency towards increases in tumor necrosis factor (TNF)-alpha and interleukin-12 p40. Significant increases in mRNA for CD4 and a trend toward increases in CD8 were also observed in imiquimod-treated patients, suggesting activation of a cell mediated immune response. Imiquimod administration was also associated with a significant decrease in viral load as measured by HPV DNA and L1 mRNA. The effects on HPV markers were accompanied by an apparent decrease in mRNA expression for markers of cell proliferation and an increase in mRNA for markers of keratinocyte differentiation and tumor suppressors. |
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Authors:
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I Arany; S K Tyring; M A Stanley; M A Tomai; R L Miller; M H Smith; D J McDermott; H B Slade |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Randomized Controlled Trial |
Journal Detail:
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Title: Antiviral research Volume: 43 ISSN: 0166-3542 ISO Abbreviation: Antiviral Res. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-11-01 Completed Date: 1999-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8109699 Medline TA: Antiviral Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 55-63 Citation Subset: IM; X |
Affiliation:
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Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, 77555-1019, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aminoquinolines / therapeutic use* Antigens, CD / genetics, metabolism Cell Differentiation Cell Division Condylomata Acuminata / drug therapy*, immunology, virology Cytokines / genetics, metabolism Double-Blind Method Female Genital Diseases, Female / drug therapy*, immunology, virology Genital Diseases, Male / drug therapy*, immunology, virology Humans Interferon Inducers / therapeutic use* Keratinocytes / pathology Male Middle Aged Papillomaviridae / genetics, isolation & purification, physiology Polymerase Chain Reaction RNA, Messenger / metabolism Viral Load |
| Chemical | |
Reg. No./Substance:
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0/Aminoquinolines; 0/Antigens, CD; 0/Cytokines; 0/Interferon Inducers; 0/RNA, Messenger; 99011-02-6/imiquimod |
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