| Enhancement of human parainfluenza virus-induced cell fusion by pradimicin, a low molecular weight mannose-binding antibiotic. | |
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MedLine Citation:
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PMID: 9403837 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oligosaccharides, especially mannose residues, expressed on the cell surface, are thought to be important for virus-induced membrane fusion. We examined the effect of mannose-binding compounds, pradimicin derivative BMY-28,864 (PRM) and concanavalin A (Con A), on cell fusion of human parainfluenza type 2 virus (hPIV2)-infected HeLa cells. Syncytium formation of hPIV2-infected HeLa cells was suppressed in the presence of Con A. On the other hand, PRM enhanced cell fusion of hPIV2-infected HeLa cells. These effects were blocked by addition of mannose-rich mannan. However, PRM shows little effect on virus growth and the expression of viral glycoproteins on the cell surface in hPIV2-infected HeLa cells. Fluorescein-isothiocyanate-labeled pradimicin and Con A bound to both uninfected and hPIV2-infected mononuclear cells, indicating that these compounds have an affinity to several cellular component(s). In contrast to Con A, PRM had little affinity to the viral glycoproteins. It is inferred from these results that the enhancement of hPIV2-induced cell fusion is probably due to the interaction between PRM and cellular component(s). |
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Authors:
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K Okamoto; T Oki; Y Igarashi; M Tsurudome; M Nishio; M Kawano; H Komada; M Ito; Y Sakakura; Y Ito |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Medical microbiology and immunology Volume: 186 ISSN: 0300-8584 ISO Abbreviation: Med. Microbiol. Immunol. Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1998-01-15 Completed Date: 1998-01-15 Revised Date: 2007-02-21 |
Medline Journal Info:
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Nlm Unique ID: 0314524 Medline TA: Med Microbiol Immunol Country: GERMANY |
Other Details:
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Languages: eng Pagination: 101-8 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Mie University School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anthracyclines* Antibiotics, Antineoplastic / metabolism, pharmacology* Carrier Proteins / metabolism Cell Fusion / drug effects Concanavalin A / pharmacology Hela Cells Humans Kinetics Mannans / pharmacology Mannose / metabolism Parainfluenza Virus 2, Human / drug effects*, growth & development, physiology* Viral Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anthracyclines; 0/Antibiotics, Antineoplastic; 0/Carrier Proteins; 0/Mannans; 0/Viral Proteins; 11028-71-0/Concanavalin A; 130743-10-1/pradimicin D; 131426-59-0/N,N-dimethylpradimicin FA-2; 131443-10-2/pradimicin FA 2; 31103-86-3/Mannose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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