Document Detail

Enhancement of human parainfluenza virus-induced cell fusion by pradimicin, a low molecular weight mannose-binding antibiotic.
MedLine Citation:
PMID:  9403837     Owner:  NLM     Status:  MEDLINE    
Oligosaccharides, especially mannose residues, expressed on the cell surface, are thought to be important for virus-induced membrane fusion. We examined the effect of mannose-binding compounds, pradimicin derivative BMY-28,864 (PRM) and concanavalin A (Con A), on cell fusion of human parainfluenza type 2 virus (hPIV2)-infected HeLa cells. Syncytium formation of hPIV2-infected HeLa cells was suppressed in the presence of Con A. On the other hand, PRM enhanced cell fusion of hPIV2-infected HeLa cells. These effects were blocked by addition of mannose-rich mannan. However, PRM shows little effect on virus growth and the expression of viral glycoproteins on the cell surface in hPIV2-infected HeLa cells. Fluorescein-isothiocyanate-labeled pradimicin and Con A bound to both uninfected and hPIV2-infected mononuclear cells, indicating that these compounds have an affinity to several cellular component(s). In contrast to Con A, PRM had little affinity to the viral glycoproteins. It is inferred from these results that the enhancement of hPIV2-induced cell fusion is probably due to the interaction between PRM and cellular component(s).
K Okamoto; T Oki; Y Igarashi; M Tsurudome; M Nishio; M Kawano; H Komada; M Ito; Y Sakakura; Y Ito
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Medical microbiology and immunology     Volume:  186     ISSN:  0300-8584     ISO Abbreviation:  Med. Microbiol. Immunol.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1998-01-15     Completed Date:  1998-01-15     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  0314524     Medline TA:  Med Microbiol Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  101-8     Citation Subset:  IM    
Department of Microbiology, Mie University School of Medicine, Japan.
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MeSH Terms
Antibiotics, Antineoplastic / metabolism,  pharmacology*
Carrier Proteins / metabolism
Cell Fusion / drug effects
Concanavalin A / pharmacology
Hela Cells
Mannans / pharmacology
Mannose / metabolism
Parainfluenza Virus 2, Human / drug effects*,  growth & development,  physiology*
Viral Proteins / metabolism
Reg. No./Substance:
0/Anthracyclines; 0/Antibiotics, Antineoplastic; 0/Carrier Proteins; 0/Mannans; 0/Viral Proteins; 11028-71-0/Concanavalin A; 130743-10-1/pradimicin D; 131426-59-0/N,N-dimethylpradimicin FA-2; 131443-10-2/pradimicin FA 2; 31103-86-3/Mannose

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