Document Detail


Enhancement of human endometrial stromal decidualization by bestatin, an aminopeptidase-N inhibitor.
MedLine Citation:
PMID:  10923279     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have re-evaluated the effects of bestatin, an aminopeptidase-N (CD13) inhibitor, on in vitro decidualization of normal human endometrial stromal cells by using an in vitro decidualization activity assay. Bestatin did not show any effects on the viability of both the decidualizing stromal cells co-stimulated with 8-Br-cAMP and bestatin and the 8-Br-cAMP-induced decidualized stromal cells, or on prolactin release from the decidualized stromal cells. However, bestatin dose-dependently enhanced prolactin release from the decidualizing stromal cells co-stimulated with 8-Br-cAMP and bestatin. These results indicate that bestatin enhances cAMP-mediated decidualization of human endometrial stromal cells and suggests that membrane aminopeptidase-N in the human endometrium is involved in the regulation of endometrial differentiation by inhibiting cAMP-mediated decidualization signals in endometrial stromal cells.
Authors:
T Tanaka; N Umesaki; M Masuda; K Mizuno; S Ogita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology     Volume:  14     ISSN:  0951-3590     ISO Abbreviation:  Gynecol. Endocrinol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2000-11-08     Completed Date:  2000-11-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8807913     Medline TA:  Gynecol Endocrinol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  182-6     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Antigens, CD13 / antagonists & inhibitors*
Cells, Cultured
Decidua / drug effects,  physiology*
Endometrium / physiology*
Enzyme Inhibitors / pharmacology*
Female
Humans
Leucine / analogs & derivatives*,  pharmacology*
Prolactin / analysis,  secretion
Stromal Cells / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 58970-76-6/bestatin; 61-90-5/Leucine; 9002-62-4/Prolactin; EC 3.4.11.2/Antigens, CD13

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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