Document Detail

Enhancement of fibroblast proliferation, collagen biosynthesis and production of growth factors as a result of combining sodium hyaluronate and aminoacids.
MedLine Citation:
PMID:  19505400     Owner:  NLM     Status:  MEDLINE    
Fibroblasts play a key role in tissue healing by producing the majority of extracellular matrix components, favouring granulation tissue formation, and stimulating re-epithelialization. Hyaluronan is a component of ECM and its anti-inflammatory effects and properties in enhancing wound closure are well known. In this study, we examined the effects of Aminogam gel, a new pharmacological preparation suggested to improve wound healing, composed of hyaluronic acid, proline, lysine, glycine and leucine, on human fibroblasts. Results show that fibroblasts treated with hyaluronic acid plus aminoacid solution increased their proliferative activity, collagen I and III, and fibronectin synthesis. Moreover, HA plus aminoacid solution increased the expression of transforming growth factor beta, connective tissue growth factor, interleukin-6 and -8, assayed by RT-PCR. These results suggested that Aminogam gel, involved in several stages of wound healing, as fibroblast proliferation, granulation tissue formation, ECM component deposition, and production of cytokines, may be a useful device to favour and accelerate wound closure.
M A Mariggiò; A Cassano; A Vinella; A Vincenti; R Fumarulo; L Lo Muzio; E Maiorano; D Ribatti; G Favia
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of immunopathology and pharmacology     Volume:  22     ISSN:  0394-6320     ISO Abbreviation:  Int J Immunopathol Pharmacol     Publication Date:    2009 Apr-Jun
Date Detail:
Created Date:  2009-06-09     Completed Date:  2009-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911335     Medline TA:  Int J Immunopathol Pharmacol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  485-92     Citation Subset:  IM    
Department of Biomedical Sciences and Human Oncology, University of Bari Medical School, Bari, Italy.
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MeSH Terms
Amino Acids / pharmacology*
Cell Cycle / drug effects
Cell Proliferation / drug effects*
Cells, Cultured
Collagen Type I / biosynthesis*,  genetics
Collagen Type III / biosynthesis*,  genetics
Connective Tissue Growth Factor / metabolism
Drug Combinations
Fibroblasts / drug effects*,  metabolism
Fibronectins / biosynthesis
Hyaluronic Acid / pharmacology*
Intercellular Signaling Peptides and Proteins / genetics,  metabolism*
Interleukin-6 / metabolism
Interleukin-8 / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Transforming Growth Factor beta / metabolism
Wound Healing / drug effects*
Reg. No./Substance:
0/Amino Acids; 0/CTGF protein, human; 0/Collagen Type I; 0/Collagen Type III; 0/Drug Combinations; 0/Fibronectins; 0/IL6 protein, human; 0/IL8 protein, human; 0/Intercellular Signaling Peptides and Proteins; 0/Interleukin-6; 0/Interleukin-8; 0/Transforming Growth Factor beta; 139568-91-5/Connective Tissue Growth Factor; 9004-61-9/Hyaluronic Acid

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