Document Detail

Enhancement of enzyme-activated 1,2-dioxetane chemiluminescence in membrane-based assays.
MedLine Citation:
PMID:  7710059     Owner:  NLM     Status:  MEDLINE    
Processes for enhancing the chemiluminescent signal generated by enzyme-activated 1,2-dioxetanes have been developed for membrane-based assays in which detection is done using a charge-coupled device (CCD) camera or X-ray film. The enhancement is demonstrated using slot-blots of biotinylated lambda DNA in conjunction with an avidin-alkaline phosphatase conjugate. For detection with a CCD camera, the nylon membrane is dried after processing and incubation in dioxetane substrate solution and heated to temperatures of 50 to 80 degrees C during detection. Up to a 100-fold signal increase is obtained using this enhancement process compared to the conventional detection procedure, in which the blot is kept saturated with substrate solution in a sealed plastic bag during detection. For detection with X-ray film, a fivefold increase in signal intensity is realized by drying the membrane before exposure to the film. These enhancement processes greatly reduce the time required for detection in membrane-based assays.
R K Kobos; B A Blue; C W Robertson; L A Kielhorn
Related Documents :
11768459 - A new calcium-sensor based on ion-selective conductometric microsensors--membranes and ...
15566149 - Electroneutrality coupling of electron transfer at an electrode surface and ion transfe...
12296569 - Optimum selection of an implantable secondary battery for an artificial heart by examin...
8011919 - A novel method for rapid measurement of membrane resistance, capacitance, and access re...
12498209 - Application of an absorption-based surface plasmon resonance principle to the developme...
10763259 - Uranyl-selective electrode based on a new bifunctional derivative combining the synergi...
22181649 - Thermodynamics of ion-containing polymer blends and block copolymers.
15713029 - Effect of temperature, elevated carbon dioxide, and drought during seed development on ...
1639199 - Do unconventional myosins exert functions in dynamics of membrane compartments?
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  224     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-05-10     Completed Date:  1995-05-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  128-33     Citation Subset:  IM    
Corporate New Business Development Division, DuPont Company, Incorporated, Wilmington, Delaware 19880.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chemiluminescent Measurements
Heterocyclic Compounds / chemistry*
Signal Processing, Computer-Assisted
X-Ray Film
Reg. No./Substance:
0/Heterocyclic Compounds; 6788-84-7/1,2-dioxetane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Oxidation of cysteine and methionine residues during acid hydrolysis of proteins in the presence of ...
Next Document:  Separation of pure populations of epithelial cells from rabbit distal colon.