Document Detail


Enhancement of enterohemorrhagic Escherichia coli O157:H7 stress tolerance via pre-heating.
MedLine Citation:
PMID:  22159372     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enterohemorrhagic Escherichia coli O157:H7 infection causes several hundred cases of food poisoning every year in Japan. In severe cases, this type of food poisoning can be fatal. In the present study, we examined the induction of HSP70 in E. coli O157:H7 cells at various temperatures and the thermotolerance of E. coli O157:H7 cells alone and in contaminated food following pre-heating. We evaluated the possibility that thermotolerance by E. coli O157:H7 increases the likelihood of food poisoning. E. coli O157:H7 cells were heated at 43-51 °C, and the survival rate was examined. The temperature of highest induction of HSP70 was used as the pre-heating temperature. We measured the thermotolerance of E. coli O157:H7 cells following pre-heating as the survival after heating at 53 °C (lethal temperature). Additionally, we evaluated the thermotolerance of E. coli O157:H7 cells in ground beef following pre-heating. Heating at 47 °C for 30 min caused the highest induction of HSP70 and this temperature was selected as the pre-heating temperature. The survival rate was significantly higher for 0-90 min compared to that in cultures incubated at 53 °C without pre-heating indicating thermotolerance. Additionally, in ground beef, thermotolerance in E. coli O157:H7 cells was induced by pre-heating. We showed that E. coli O157:H7 cells acquired thermotolerance after pre-heating, which significantly increased survival after a lethal temperature, and increased the likelihood of food poisoning.
Authors:
Masanori Nakano; Youko Itoh; Yoshiaki Yamada; Kogenta Nakamura; Makoto Sumitomo; Masakazu Nitta
Publication Detail:
Type:  Journal Article     Date:  2011-12-09
Journal Detail:
Title:  Molecular medicine reports     Volume:  5     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-01-05     Completed Date:  2012-05-03     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  715-20     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Division of Hematology, Aichi Medical University School of Medicine, Aichi 480-1195, Japan. m.nakano@apha.jp
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Escherichia coli O157 / growth & development,  metabolism*
Escherichia coli Proteins / metabolism
Foodborne Diseases / microbiology
Gene Expression Regulation, Bacterial / physiology
HSP70 Heat-Shock Proteins / metabolism
Hot Temperature
Humans
Meat / microbiology
Stress, Physiological / physiology
Chemical
Reg. No./Substance:
0/Escherichia coli Proteins; 0/HSP70 Heat-Shock Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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