Document Detail

Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two-stage oxygen supply control strategy based on oxygen transfer coefficient.
MedLine Citation:
PMID:  21070268     Owner:  NLM     Status:  In-Data-Review    
Aims:  To improve the yield and productivity of docosahexaenoic acid (DHA) by Schizochytrium sp. in terms of the analysis of microbial physiology. Methods and Results:  A two-stage oxygen supply control strategy, aimed at achieving high concentration and high productivity of DHA, was proposed. At the first 40 h, K(L) a was controlled at 150·1 h(-1) to obtain high μ for cell growth, subsequently K(L) a was controlled at 88·5 h(-1) to maintain high q(p) for high DHA accumulation. Finally, the maximum lipid, DHA content and DHA productivity reached 46·6, 17·7 g l(-1) and 111 mg l(-1)  h(-1) , which were 43·83%, 63·88% and 32·14% over the best results controlled by constant K(L) a. Conclusions:  This paper described a two-stage oxygen supply control strategy based on the kinetic analysis for efficient DHA fermentation by Schizochytrium sp. Significance and Impact of the study:  This study showed the advantage of two-stage control strategy in terms of microbial physiology. As K(L) a is a scaling-up parameter, the idea developed in this paper could be scaled-up to industrial process and applied to other industrial biotechnological processes to achieve both high product concentration and high productivity.
L Qu; X-J Ji; L-J Ren; Z-K Nie; Y Feng; W-J Wu; P-K Ouyang; H Huang
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Publication Detail:
Type:  Journal Article     Date:  2010-11-12
Journal Detail:
Title:  Letters in applied microbiology     Volume:  52     ISSN:  1472-765X     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  22-7     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Letters in Applied Microbiology © 2010 The Society for Applied Microbiology.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.
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