| Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two-stage oxygen supply control strategy based on oxygen transfer coefficient. | |
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MedLine Citation:
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PMID: 21070268 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Aims: To improve the yield and productivity of docosahexaenoic acid (DHA) by Schizochytrium sp. in terms of the analysis of microbial physiology. Methods and Results: A two-stage oxygen supply control strategy, aimed at achieving high concentration and high productivity of DHA, was proposed. At the first 40 h, K(L) a was controlled at 150·1 h(-1) to obtain high μ for cell growth, subsequently K(L) a was controlled at 88·5 h(-1) to maintain high q(p) for high DHA accumulation. Finally, the maximum lipid, DHA content and DHA productivity reached 46·6, 17·7 g l(-1) and 111 mg l(-1) h(-1) , which were 43·83%, 63·88% and 32·14% over the best results controlled by constant K(L) a. Conclusions: This paper described a two-stage oxygen supply control strategy based on the kinetic analysis for efficient DHA fermentation by Schizochytrium sp. Significance and Impact of the study: This study showed the advantage of two-stage control strategy in terms of microbial physiology. As K(L) a is a scaling-up parameter, the idea developed in this paper could be scaled-up to industrial process and applied to other industrial biotechnological processes to achieve both high product concentration and high productivity. |
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Authors:
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L Qu; X-J Ji; L-J Ren; Z-K Nie; Y Feng; W-J Wu; P-K Ouyang; H Huang |
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Publication Detail:
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Type: Journal Article Date: 2010-11-12 |
Journal Detail:
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Title: Letters in applied microbiology Volume: 52 ISSN: 1472-765X ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 22-7 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Letters in Applied Microbiology © 2010 The Society for Applied Microbiology. |
Affiliation:
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State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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