Document Detail


Enhancement of the cytotoxicity of mistletoe lectin-1 (ML-1) by high pH or perturbation in Golgi functions.
MedLine Citation:
PMID:  1910176     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have studied the cytotoxicity of Mistletoe lectin-1 (ML-1), a cytotoxic protein produced by Viscum album, in CHO and V79 cells and in mutant cell lines altered in Golgi functions or in endosomal acidification. In wild-type CHO cells, cytotoxicity of ML-1 was greatly enhanced by ammonium chloride or nigericin. A CHO mutant defective in endosomal acidification (DMPR-2), which is resistant to diphtheria toxin, modeccin and Pseudomonas aeruginosa exotoxin A and hypersensitive to ricin, showed increased sensitivity to ML-1. MonR-31 and MF-1 are monensin- and compactin-resistant mutants derived from CHO and V79 cell lines, respectively, and are presumably altered in Golgi functions. The cytotoxicity of ML-1 was found to be increased in both MonR-31 and MF-1 cells as compared with their parental cells. These results indicate that the effects of chemicals or mutations altering endosomal acidification and Golgi functions on the cytotoxicity of ML-1 are similar to those on ricin cytotoxicity. Our results suggest that the cytotoxicity of ML-1 is enhanced by an increase in endosomal pH, as well as by chemicals or mutations altering the structure/functions of the Golgi regions. Like ricin, the intoxication process of ML-1 may involve the Golgi regions.
Authors:
T Yoshida; M Zhang; C Chen; H Franz; H C Wu
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Die Pharmazie     Volume:  46     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-10-18     Completed Date:  1991-10-18     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  349-51     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland.
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MeSH Terms
Descriptor/Qualifier:
Ammonium Chloride / pharmacology
Animals
Cell Survival / drug effects*
Cricetinae
Cricetulus
Female
Golgi Apparatus / drug effects*
Hydrogen-Ion Concentration
Mutation
Nigericin / pharmacology
Ovary / cytology
Plant Preparations*
Plant Proteins*
Pseudomonas aeruginosa / drug effects
Ribosome Inactivating Proteins, Type 2
Ricin / toxicity
Toxins, Biological / pharmacology*
Vero Cells / drug effects
Grant Support
ID/Acronym/Agency:
GM28810/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Plant Preparations; 0/Plant Proteins; 0/Ribosome Inactivating Proteins, Type 2; 0/Toxins, Biological; 0/ribosome inactivating protein, Viscum; 12125-02-9/Ammonium Chloride; 28380-24-7/Nigericin; 9009-86-3/Ricin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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