| Enhancement of the cytotoxicity of mistletoe lectin-1 (ML-1) by high pH or perturbation in Golgi functions. | |
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MedLine Citation:
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PMID: 1910176 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have studied the cytotoxicity of Mistletoe lectin-1 (ML-1), a cytotoxic protein produced by Viscum album, in CHO and V79 cells and in mutant cell lines altered in Golgi functions or in endosomal acidification. In wild-type CHO cells, cytotoxicity of ML-1 was greatly enhanced by ammonium chloride or nigericin. A CHO mutant defective in endosomal acidification (DMPR-2), which is resistant to diphtheria toxin, modeccin and Pseudomonas aeruginosa exotoxin A and hypersensitive to ricin, showed increased sensitivity to ML-1. MonR-31 and MF-1 are monensin- and compactin-resistant mutants derived from CHO and V79 cell lines, respectively, and are presumably altered in Golgi functions. The cytotoxicity of ML-1 was found to be increased in both MonR-31 and MF-1 cells as compared with their parental cells. These results indicate that the effects of chemicals or mutations altering endosomal acidification and Golgi functions on the cytotoxicity of ML-1 are similar to those on ricin cytotoxicity. Our results suggest that the cytotoxicity of ML-1 is enhanced by an increase in endosomal pH, as well as by chemicals or mutations altering the structure/functions of the Golgi regions. Like ricin, the intoxication process of ML-1 may involve the Golgi regions. |
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Authors:
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T Yoshida; M Zhang; C Chen; H Franz; H C Wu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Die Pharmazie Volume: 46 ISSN: 0031-7144 ISO Abbreviation: Pharmazie Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-10-18 Completed Date: 1991-10-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9800766 Medline TA: Pharmazie Country: GERMANY |
Other Details:
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Languages: eng Pagination: 349-51 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ammonium Chloride
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pharmacology Animals Cell Survival / drug effects* Cricetinae Cricetulus Female Golgi Apparatus / drug effects* Hydrogen-Ion Concentration Mutation Nigericin / pharmacology Ovary / cytology Plant Preparations* Plant Proteins* Pseudomonas aeruginosa / drug effects Ribosome Inactivating Proteins, Type 2 Ricin / toxicity Toxins, Biological / pharmacology* Vero Cells / drug effects |
| Grant Support | |
ID/Acronym/Agency:
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GM28810/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Plant Preparations; 0/Plant Proteins; 0/Ribosome Inactivating Proteins, Type 2; 0/Toxins, Biological; 0/ribosome inactivating protein, Viscum; 12125-02-9/Ammonium Chloride; 28380-24-7/Nigericin; 9009-86-3/Ricin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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