Document Detail


Enhancement of the blood growth promoting activity after exposure of volunteers to visible and infrared polarized light. Part I: stimulation of human keratinocyte proliferation in vitro.
MedLine Citation:
PMID:  14743286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The systemic mechanisms of the wound healing effect of low intensity lasers remain largely uninvestigated. The goal of this randomized, placebo controlled, double blind study is to prove that irradiation of a small area of the human body with visible and infrared polarized (VIP) light (400-3400 nm, 95% polarization, 40 mW cm(2), 12 J cm(2)) leads to an increase of the growth promoting (GP) activity of the entire circulating blood for primary cultures of human keratinocytes (KCs). Thirty minutes after the VIP-irradiation of a sacral area of volunteers, the GP activity of circulating blood was seen to increase through the elevation of the number of KCs cultured with the isolated plasma by 20 +/- 3%, p < 0.001. A similar increase in GP activity was seen in plasma derived from the in vitro irradiated blood of each volunteer, and from the mixture of irradiated and non-irradiated autologous blood 1:10. Enhanced GP activity was also recorded at 24 h after the 1st and 4-9th daily phototherapeutic sessions. Hence, exposure of volunteers to VIP light leads to a fast increase in the GP activity of the entire circulating blood for human KCs in vitro, which is a consequence of the transcutaneous photomodification of blood and its effect on the rest of the circulating blood volume.
Authors:
Kira A Samoilova; Olga N Bogacheva; Ksenya D Obolenskaya; Miralda I Blinova; Natalya V Kalmykova; Elena V Kuzminikh
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Publication Detail:
Type:  Clinical Trial; Comparative Study; In Vitro; Journal Article; Randomized Controlled Trial     Date:  2003-09-01
Journal Detail:
Title:  Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology     Volume:  3     ISSN:  1474-905X     ISO Abbreviation:  Photochem. Photobiol. Sci.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2004-01-26     Completed Date:  2004-08-06     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  101124451     Medline TA:  Photochem Photobiol Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  96-101     Citation Subset:  IM    
Affiliation:
Institute of Cytology, Russian Academy of Sciences, 4 Tikhoretsky Ave, Saint Petersburg, 194064, Russia. kirasam@mail.cytspb.rssi.ru
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Cell Division / radiation effects*
Dose-Response Relationship, Radiation
Female
Growth Substances / blood*,  radiation effects*
Humans
Infrared Rays*
Keratinocytes / cytology,  radiation effects*
Light*
Male
Middle Aged
Chemical
Reg. No./Substance:
0/Growth Substances

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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