Document Detail


Enhancement of bacillus Calmette-Guerin attachment to the urothelium by removal of the rabbit bladder mucin layer.
MedLine Citation:
PMID:  1732628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been established that the urothelial mucin layer functions as a bacterial anti-adherence factor. Intravesical Bacillus Calmette-Guerin is used to treat patients with superficial bladder cancer. The proposed mechanism of action of Bacillus Calmette-Guerin is adherence to the urothelium with induction of an immunologic and/or inflammatory response. The current study was designed to determine if rabbit bladder mucin removal results in increased Bacillus Calmette-Guerin urothelial adherence. PAS and colloidal iron stains were used to demonstrate that intravesical instillation of 50% acetone renders rabbit bladder urothelium mucin deficient. The urothelium remains mucin deficient at two hours, but by 24 hours the mucin layer has been regenerated. Two hours following intravesical 3H-labeled Escherichia coli administration, bacterial adherence was 29-fold greater in mucin deficient than mucin intact rabbits (p = 0.05). By 12 hours, the difference in adherence was not significant. Two hours following intravesical administration of 3H-labeled Bacillus Calmette Guerin, mucosal adherence was 21-fold greater in mucin deficient compared to mucin intact rabbits (p = 0.002). After mucin removal, Bacillus Calmette Guerin urothelial adherence was significantly increased. The significant increase in Bacillus Calmette Guerin adherence after mucin removal may be clinically exploitable.
Authors:
R A Badalament; G L Franklin; C M Page; B M Dasani; M G Wientjes; J R Drago
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of urology     Volume:  147     ISSN:  0022-5347     ISO Abbreviation:  J. Urol.     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-02-25     Completed Date:  1992-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376374     Medline TA:  J Urol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  482-5     Citation Subset:  AIM; IM    
Affiliation:
Department of Surgery, Ohio State University, Columbus.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Adhesion*
Epithelium / microbiology
Escherichia coli / physiology
Male
Mucins / physiology*
Mycobacterium bovis / physiology*
Rabbits
Urinary Bladder / microbiology*
Chemical
Reg. No./Substance:
0/Mucins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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