| Enhancement of anchorage-independent growth of a human adrenal carcinoma cell line by endogenously produced basic fibroblast growth factor. | |
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MedLine Citation:
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PMID: 2168346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously observed that a human adrenal carcinoma cell line (SW-13) produces autocrine growth factors that enhance the ability of these cells to clone in soft agar. We now show that a basic FGF-like protein is found in SW-13 lysates and stimulates SW-13 colony growth. Basic FGF (bFGF) was identified in SW-13 lysates by both its chromatographic behavior on heparin-Sepharose and its reactivity with an antibody specific for bFGF. Incubation of lysates with antibodies specific for bFGF abolished their ability to support anchorage-independent growth. Northern analysis demonstrated that the cells produced multiple bFGF mRNA transcripts. SW-13 cells also expressed bFGF cell-surface receptors. These data suggest that SW-13 cells produce and respond to a factor structurally and functionally related to bFGF. Such findings support recent evidence indicating that FGF secreted by human tumor cell lines plays a role in the maintenance of the transformed phenotype. |
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Authors:
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S J Corin; L C Chen; A W Hamburger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: International journal of cancer. Journal international du cancer Volume: 46 ISSN: 0020-7136 ISO Abbreviation: Int. J. Cancer Publication Date: 1990 Sep |
Date Detail:
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Created Date: 1990-10-09 Completed Date: 1990-10-09 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0042124 Medline TA: Int J Cancer Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 516-21 Citation Subset: IM |
Affiliation:
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Cancer Center, University of Maryland, Baltimore, MD 21201. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Gland Neoplasms
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metabolism*,
pathology Antibodies / immunology Blotting, Northern Cell Division / drug effects* Colony-Stimulating Factors / immunology Fibroblast Growth Factors / genetics, immunology, metabolism* Humans Models, Biological Neoplastic Stem Cells / metabolism RNA, Messenger / isolation & purification Receptors, Cell Surface / analysis Receptors, Fibroblast Growth Factor Tumor Cells, Cultured / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA48193/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Colony-Stimulating Factors; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, Fibroblast Growth Factor; 62031-54-3/Fibroblast Growth Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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