| Enhancement of activity and selectivity of Candida rugosa lipase and Candida antarctica lipase A by bioimprinting and/or immobilization for application in the selective ethanolysis of fish oil. | |
| | |
MedLine Citation:
|
PMID: 21695486 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Candida rugosa lipase (CRL) and Candida antarctica lipase A (CALA) with improved activity and selectivity were prepared for use in organic solvent media. CRL bioimprinted with fatty acids exhibited eightfold enhanced transesterification activity in hexane. Combination of bioimprinting and coating with lecithin or with immobilization did not improve the activity further. CALA was immobilized with and without bioimprinting, none of which improved the activity. All modified lipases were tested for selective ethanolysis of fish oil to concentrate omega-3 polyunsaturated fatty acids (PUFA). None of the preparations, except the immobilized ones catalysed ethanolysis. Immobilized CRL-catalyzed ethanolysis giving 27% (v/v) ethyl esters (EE) in 48 h, of which 43 mol% was oleic acid but no PUFA was detected in the EE fraction. Fatty acid selectivity of CALA was significantly improved by immobilization combined with bioimprinting, resulting in 5.5-fold lower omega-3 PUFA in EE. |
| | |
Authors:
|
Derya Kahveci; Xuebing Xu |
Related Documents
:
|
8889486 - 2d nmr of the metabolic antioxidant dihydrolipoic acid and its derivatives. 8962446 - 2-naphthoic acid at 153 k. 17497776 - Anion chelation by amido acid functionalized fused quartz/water interfaces studied by n... 11975536 - Exploring the active site of amine:pyruvate aminotransferase on the basis of the substr... 882826 - Gastric acid secretion in patients with symptomatic hiatus hernia and effect of a modif... 6482386 - Type ii epithelial cells of the lung. vii. the effect of ascorbic acid and glutathione. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-22 |
Journal Detail:
|
Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-22 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10, 8000, Aarhus C, Denmark, dek@mb.au.dk. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Using clinical cardiovascular risk scores to predict coronary artery plaque severity and stenosis de...
Next Document: A highly stable Cu/Zn superoxide dismutase from Withania somnifera plant: gene cloning, expression a...