Document Detail


Enhancement of the activation of Glu-plasminogen by urokinase in the simultaneous presence of tranexamic acid or fibrin.
MedLine Citation:
PMID:  2920977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activation rate of Glu-plasminogen (Glu-plg) by urokinase (UK) was enhanced in the presence of either tranexamic acid or fibrin with an increase in the catalytic rate constant (kcat). The maximum increase in kcat was obtained at 0.5 mM of tranexamic acid and 0.1 microM of fibrin. Km did not change. The addition of fibrin to 1 mM tranexamic acid resulted in a further increase in kcat of the UK activation of Glu-plg. On the other hand, the addition of tranexamic acid to 0.1 microM fibrin further increased kcat of the UK activation of Glu-plg. Thus, stimulatory effects were observed on the activation of Glu-plg by UK in the simultaneous presence of tranexamic acid and fibrin. Fibrin-binding sites on the kringle 5 of Glu-plg may be involved in the further increase in the activation rate of Glu-plg by UK in the presence of both fibrin and tranexamic acid in comparison to that in the presence of tranexamic acid alone. Possibly, the Glu-plg binding with both tranexamic acid and fibrin (at kringle 5) may be most effectively activated by UK. It is also suggested that two molecules of Glu-plg bind to one molecule of fibrin monomer.
Authors:
A Takada; Y Sugawara; Y Takada
Related Documents :
24138287 - Caffeic acid induces keratinocyte differentiation by activation of ppar-α
15826417 - The use of tranexamic acid to reduce blood loss during total hip arthroplasty: an obser...
24335967 - Safety assessment of α-amino acids as used in cosmetics.
16470437 - Tranexamic acid reduces blood loss in cemented hip arthroplasty: a randomized, double-b...
2665567 - Vitamin k (menaquinone) biosynthesis in bacteria: high-performance liquid chromatograph...
6768717 - Autolysis of escherichia coli.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Haemostasis     Volume:  19     ISSN:  0301-0147     ISO Abbreviation:  Haemostasis     Publication Date:  1989  
Date Detail:
Created Date:  1989-04-11     Completed Date:  1989-04-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0371574     Medline TA:  Haemostasis     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  26-31     Citation Subset:  IM    
Affiliation:
Department of Physiology, Hamamatsu University, School of Medicine, Shizuoka-ken, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cyclohexanecarboxylic Acids / pharmacology*
Drug Synergism
Enzyme Activation / drug effects
Fibrin / pharmacology*
Humans
Kinetics
Plasminogen / metabolism*
Protein Binding
Tranexamic Acid / pharmacology*
Urokinase-Type Plasminogen Activator / pharmacology*
Chemical
Reg. No./Substance:
0/Cyclohexanecarboxylic Acids; 1197-18-8/Tranexamic Acid; 9001-31-4/Fibrin; 9001-91-6/Plasminogen; EC 3.4.21.73/Urokinase-Type Plasminogen Activator

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Intraperitoneal heparin in peritoneal dialysis and its effect on fibrinopeptide A in plasma and dial...
Next Document:  Protein C in the neonatal period.