| Enhancement of Vindoline Production in Suspension Culture of the Catharanthus roseus Cell Line C20hi by Light and Methyl Jasmonate Elicitation. | |
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MedLine Citation:
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PMID: 22156254 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The effects of light and methyl jasmonate (MJ) on the transcription of biosynthetic genes as well as the accumulation of vindoline and catharanthine in Catharanthus roseus C20hi cell suspensions were studied. t16h (the gene encoding tabersonine 16-hydroxylase) could be induced by light and MJ, whereas d4h (the gene encoding deacetoxyvindoline 4-hydroxylase) could only be induced by light. Quantification by UPLC-MS showed that light significantly increased vindoline production in C20hi cells by about 0.49 - 5.51-fold more than that in controls, with the highest yield being 75.3 ng/g of dry weight. The biosynthesis of vindoline was further enhanced by combining MJ with light. The accumulation of catharanthine was not improved by either light or MJ elicitation. These results suggested that light and MJ could promote vindoline, but not catharanthine accumulation in C20hi cells. |
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Authors:
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Lihong He; Li Yang; Ronghui Tan; Shujuan Zhao; Zhibi Hu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 27 ISSN: 1348-2246 ISO Abbreviation: Anal Sci Publication Date: 2011 |
Date Detail:
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Created Date: 2011-12-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 1243 Citation Subset: IM |
Affiliation:
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The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicines, The Shanghai Key Laboratory for Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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