Document Detail


Enhancement of TAT cell membrane penetration efficiency by dimethyl sulphoxide.
MedLine Citation:
PMID:  20025914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell penetrating peptides (CPPs) are promising tools for transducing presynthesized therapeutic molecules which possess low membrane permeability. The poor efficiency of cellular uptake and unexpected cellular localization are still the main obstacles to the development of drug delivery by using CPPs. In this study, we investigated the effect of a penetration enhancer, dimethylsulfoxide (DMSO), on the penetrating efficiency of a synthetic TAT peptide or the TAT fusion protein. FITC-labeled TAT and TAT-GFP were added to 10% DMSO or 100 microM chloroquine pretreated cells, fluorescence uptake into culturing cells was observed using fluorescence microscopy, FACS or quantitatively analyzed by a fluorescence spectrum. 10% DMSO treatment markedly increased internalization of TAT into cells and appeared in a well-distributed pattern throughout the cytosol and nucleus without membrane perforating or detectable cytotoxicity, the enhancement effect by 10% DMSO was reduced by endocytosis inhibitors including ammonium chloride and sodium azide. 10% DMSO also enhanced TAT-Apoptin induced apoptosis of carcinoma cells. These findings implicated that DMSO can be a novel delivery enhancer appropriate for CPP penetration.
Authors:
Hu Wang; Chun-Yan Zhong; Jiang-Feng Wu; Yu-Bin Huang; Chang-Bai Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-16
Journal Detail:
Title:  Journal of controlled release : official journal of the Controlled Release Society     Volume:  143     ISSN:  1873-4995     ISO Abbreviation:  J Control Release     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-16     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607908     Medline TA:  J Control Release     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  64-70     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
The Institute of Molecular Biology, Three Georges University, Yichang, 443002, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Active Transport, Cell Nucleus
Ammonium Chloride / pharmacology
Animals
Apoptosis / drug effects
Capsid Proteins / genetics,  metabolism
Cell Membrane / drug effects*,  metabolism
Cell Membrane Permeability / drug effects*
Cell Nucleus / metabolism
Cell Separation / methods
Chloroquine / pharmacology
Cytosol / metabolism
Dimethyl Sulfoxide / pharmacology*
Dose-Response Relationship, Drug
Endocytosis / drug effects
Flow Cytometry
Fluorescein-5-isothiocyanate / metabolism
Green Fluorescent Proteins / genetics,  metabolism
Hemolysis / drug effects
Hep G2 Cells
Humans
Mice
Mice, Inbred BALB C
Microscopy, Fluorescence
Recombinant Fusion Proteins / metabolism
Sodium Azide / pharmacology
Time Factors
tat Gene Products, Human Immunodeficiency Virus / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Capsid Proteins; 0/Recombinant Fusion Proteins; 0/VP3 protein, Chicken anemia virus; 0/tat Gene Products, Human Immunodeficiency Virus; 12125-02-9/Ammonium Chloride; 147336-22-9/Green Fluorescent Proteins; 26628-22-8/Sodium Azide; 3326-32-7/Fluorescein-5-isothiocyanate; 54-05-7/Chloroquine; 67-68-5/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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