| Enhancement of shrimp antiviral immune response through caspase-dependent apoptosis by small molecules. | |
| | |
MedLine Citation:
|
PMID: 20936319 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Epidemic diseases cost large amount of economic loss in the shrimp aquaculture. To control the epidemic diseases, it is a very efficient approach to enhance the shrimp immunity by immunostimulants. In aquaculture, however, the applications of the available immunostimulants are very limited due to the lack of information about the roles of these immunostimulants in animal immunity. In the present study, a caspase protein (PjCaspase), required in shrimp antiviral apoptosis, was used as the target protein to screen for small molecules which would enhance the shrimp immunity. Based on screening using the EGFP-PjCaspase fusion protein in insect cells, four small molecules could enhance the activity of PjCaspase protein. Among them, IL-2 and evodiamine were further evidenced to enhance the apoptotic activity of shrimp hemocytes in vivo, suggesting that the small molecules improved the activity of apoptosis through the activation of the PjCaspase protein. The results indicated that the enhancement of apoptotic activity effectively inhibited the white spot syndrome virus (WSSV) infection in shrimp, which further led to the decrease of mortalities of WSSV-infected shrimp. Therefore, our study, for the first time, presented that the strategy using the key proteins in immune responses of aquatic organisms as the target proteins was a very efficient approach for the screening of immunostimulants to prevent the aquatic organisms from pathogen infections. |
| | |
Authors:
|
Bin Zhi; Wen Tang; Xiaobo Zhang |
Related Documents
:
|
16177559 - Bioluminescent imaging of trail-induced apoptosis through detection of caspase activati... 15662549 - Antioxidative treatment prevents activation of death-receptor- and mitochondrion-depend... 18321989 - Cleavage of mcd1 by caspase-like protease esp1 promotes apoptosis in budding yeast. 10744739 - Structure, expression, and function of the xenopus laevis caspase family. 17003409 - Differential mechanisms of conjunctival cell death induction by ultraviolet irradiation... 11058599 - Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demo... 18591139 - Nigral neurons are likely to die of a mechanism other than classical apoptosis in parki... 12677239 - The role of tarc in the pathogenesis of allergic asthma. 3156919 - Subclass specificity of fc gamma receptor-mediated inhibition of mouse b cell activation. |
Publication Detail:
|
Type: Journal Article Date: 2010-10-09 |
Journal Detail:
|
Title: Marine biotechnology (New York, N.Y.) Volume: 13 ISSN: 1436-2236 ISO Abbreviation: Mar. Biotechnol. Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-06-01 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100892712 Medline TA: Mar Biotechnol (NY) Country: United States |
Other Details:
|
Languages: eng Pagination: 575-83 Citation Subset: IM |
Affiliation:
|
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A Reverse Genetics System for the Great Lakes Strain of Viral Hemorrhagic Septicemia Virus: the NV G...
Next Document: Identification of Copper-Induced Genes in the Marine Alga Ulva compressa (Chlorophyta).