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Enhancement of Secretory Aspartyl Protease production in biofilms of Candida albicans exposed to sub-inhibitory concentrations of fluconazole.
MedLine Citation:
PMID:  19878458     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The production of Secretory Aspartyl Proteases (Sap) is an important virulence factor of Candida albicans. Many studies have shown that a challenge with sub-inhibitory concentrations of antifungals lead species of Candida to the secretion of higher concentrations of Sap. Nevertheless, published studies only reported the secretion of such enzymes by cells growing in planktonic phase, with few mention of biofilms. The present study evaluated the alterations in the secretion of Sap by C. albicans grown in biofilms and exposed to sub-inhibitory concentrations of fluconazole. The MICs for fluconazole of seven clinical strains were determined for planktonic cells. Biofilm and planktonic cells were grown in the presence of ½ MIC, ¼ MIC, and no medication (control). The relative metabolic activity, indirectly related to cell loads, were estimated by the absorbance of reduced XTT and the Sap activity was evaluated by bovine albumin test. It was observed that 72 h-old biofilms under the influence of ½ MIC had fewer cells than ¼ MIC and control. The production of Sap was inversely proportional to the cell content, with higher secretion in ½ MIC, followed by ¼ MIC and control. Biofilms of C. albicans challenged by sub-MICs of fluconazole tend to secrete higher quantities of Sap.
Authors:
Alinne Ulbrich Mores; Roberta Djavana Souza; Laura Cavalca; Alessandra de Paula E Carvalho; Lauren Christine Gursky; Rosimeire Takaki Rosa; Lakshman Perera Samaranayake; Edvaldo Antonio Ribeiro Rosa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mycoses     Volume:  54     ISSN:  1439-0507     ISO Abbreviation:  Mycoses     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8805008     Medline TA:  Mycoses     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Copyright Information:
© 2009 Blackwell Verlag GmbH.
Affiliation:
The Pontifical Catholic University of Paraná, Laboratory of Stomatology, Curitiba, PR, Brazil The Forsyth Institute, Department of Periodontology, Boston, MA, USA Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, Hong Kong, China.
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