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Enhancement of Iron(II)-dependent Reduction of Nitrite to Nitric Oxide by Thiocyanate and Accumulation of Iron(II)/Thiocyanate/Nitric Oxide Complex under the Conditions Simulating the Mixture of Saliva and Gastric Juice.
MedLine Citation:
PMID:  22145785     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Iron(III) ingested as a food component or supplement for iron deficiencies can react with salivary SCN- to produce Fe(SCN)2+ and can be reduced to iron(II) by ascorbic acid in the stomach. Iron(II) generated in the stomach can react with salivary nitrite and SCN- to produce nitric oxide (NO) and FeSCN+, respectively. The purpose of this investigation is to make clear the reactions among nitrite, SCN-, iron ions, and ascorbic acid under the conditions simulating the mixture of saliva and gastric juice. Iron(II)-dependent reduction of nitrite to NO was enhanced by SCN- in acidic buffer solutions, and the oxidation product of iron(II) reacted with SCN- to produce Fe(SCN)2+. Almost all of NO produced was autoxidized to N2O3 under aerobic conditions. Iron(II)-dependent production of NO was also observed in acidified saliva. Under anaerobic conditions, NO transformed Fe(SCN)2+ and FeSCN+ to Fe(SCN)NO+ in acidic buffer solutions. Fe(SCN)NO+ was also formed under aerobic conditions when excess ascorbic acid was added to iron(II)/nitrite/SCN- systems in acidic buffer solutions and acidified saliva. The Fe(SCN)NO+ formed was transformed to Fe(SCN)2+ and iron(III) at pH 2.0 and pH 7.4, respectively, by O2. Salivary glycoproteins could complex with iron(III) in the stomach preventing the formation of Fe(SCN)2+. Ascorbic acid reduced iron(III) to iron(II) to react with nitrite and SCN- as described above. The above results suggest (i) that iron(II) can give toxic effects to the stomach through the formation of reactive nitrogen oxide species from NO when supplemented without ascorbic acid, whereas through the formation of both reactive nitrogen oxide species and Fe(SCN)NO+ when supplemented with ascorbic acid, and (ii) that the toxic effects of iron(III) seemed to be smaller than and similar to those of iron(II) when supplemented without and with ascorbic acid, respectively. Possible mechanisms to give oxidative stresses to the stomach through Fe(SCN)NO+ are discussed.
Authors:
Umeo Takahama; Sachiko Hirota
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-6
Journal Detail:
Title:  Chemical research in toxicology     Volume:  -     ISSN:  1520-5010     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-7     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807448     Medline TA:  Chem Res Toxicol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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