| Enhancement of human gamma-interferon production in recombinant E. coli using batch cultivation. | |
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MedLine Citation:
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PMID: 19655276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Development of inexpensive and simple culture media and appropriate induction conditions are always favorable for industry. In this research, chemical composition and stoichiometric data for gamma-interferon production and recombinant Escherichia coli growth were used in order to achieve a simple medium and favorable induction conditions. To achieve this goal, the effects of medium composition and induction conditions on the production of gamma-interferon were investigated in batch culture of E. coli BL21 (DE3) [pET3a-ifngamma]. These conditions were considered as suitable conditions for the production of gamma-interferon: 2.5x M9 medium, supplemented with a mixture of amino acids (milligram per liter), including glutamic acid 215, aspartic acid 250, lysine 160, and phenylalanine 90, and induction at late-log phase (OD(600) = 4.5). Under these conditions, dry cell weight of 6 +/- 0.2 g/l and gamma-interferon concentration of 2.15 +/- 0.1 g/l were obtained. Later, without changing the concentration ratio of amino acids and glucose, the effect of increase in the primary glucose concentration on productivity of gamma-interferon was investigated. It was found that 25 g/l glucose will result in maximum attainable biomass and recombinant human gamma-interferon. At improved conditions, a dry cell weight of 14 +/- 0.2 g/l, concentration and overall productivity of gamma-interferon 4.2 +/- 0.1 g/l and 420 +/- 10 mg/l h, respectively, were obtained. |
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Authors:
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Valiollah Babaeipour; Seyed Abbas Shojaosadati; Rasoul Khalilzadeh; Nader Maghsoudi; Amir Mohammad Farnoud |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-05 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 160 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-18 Completed Date: 2010-06-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 2366-76 Citation Subset: IM |
Affiliation:
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Group of Biotechnology, Department of Chemical Engineering, Faculty of Engineering, University of Tarbiat Modares, PO Box 14115-143, Tehran, Iran. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bioreactors* Cell Culture Techniques* Culture Media / chemistry Escherichia coli* / genetics, metabolism Glucose / metabolism Humans Interferon-gamma, Recombinant / chemical synthesis*, chemistry, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Interferon-gamma, Recombinant; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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