| Enhancement of fumaric acid production by Rhizopus oryzae using a two-stage dissolved oxygen control strategy. | |
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MedLine Citation:
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PMID: 19936636 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Batch fermentative production of fumaric acid by Rhizopus oryzae ME-F12 was investigated in a 7-l stirred tank fermentor under different dissolved oxygen (DO) concentrations. High fumaric acid yield on glucose (0.56 g/g) was achieved under high DO concentration (80%), but the glucose consumption rate and fumaric acid productivity were rather low (0.91 and 0.51 g/l/h). Fumaric acid productivity was enhanced under low DO concentration (30%), but the fuamric acid yield on glucose decreased to 0.52 g/g. In order to achieve the high fumaric acid yield and productivity simultaneously, a two-stage dissolved oxygen control strategy was proposed, in which the DO concentration was controlled at 80% in the first 18 h and then switched to 30%. This was experimentally proven to be successful. Relatively high fumaric acid production (56.2 g/l), high fumaric acid yield on glucose (0.54 g/g), and high glucose consumption rate (1.3 g/l/h) were achieved by applying this strategy. The productivity (0.7 g/l/h) was improved by 37%, 21%, and 9%, respectively, compared with fermentations in which DO concentrations were kept constant at 80%, 60%, and 30%. |
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Authors:
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Yong-Qian Fu; Shuang Li; Yao Chen; Qing Xu; He Huang; Xiao-Yan Sheng |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-21 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 162 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-13 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 1031-8 Citation Subset: IM |
Affiliation:
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College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors
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microbiology Culture Techniques / methods* Fermentation Fumarates / metabolism* Glucose / metabolism Oxygen / metabolism* Rhizopus / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Fumarates; 110-17-8/fumaric acid; 50-99-7/Glucose; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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