Document Detail

Enhancement of fumaric acid production by Rhizopus oryzae using a two-stage dissolved oxygen control strategy.
MedLine Citation:
PMID:  19936636     Owner:  NLM     Status:  MEDLINE    
Batch fermentative production of fumaric acid by Rhizopus oryzae ME-F12 was investigated in a 7-l stirred tank fermentor under different dissolved oxygen (DO) concentrations. High fumaric acid yield on glucose (0.56 g/g) was achieved under high DO concentration (80%), but the glucose consumption rate and fumaric acid productivity were rather low (0.91 and 0.51 g/l/h). Fumaric acid productivity was enhanced under low DO concentration (30%), but the fuamric acid yield on glucose decreased to 0.52 g/g. In order to achieve the high fumaric acid yield and productivity simultaneously, a two-stage dissolved oxygen control strategy was proposed, in which the DO concentration was controlled at 80% in the first 18 h and then switched to 30%. This was experimentally proven to be successful. Relatively high fumaric acid production (56.2 g/l), high fumaric acid yield on glucose (0.54 g/g), and high glucose consumption rate (1.3 g/l/h) were achieved by applying this strategy. The productivity (0.7 g/l/h) was improved by 37%, 21%, and 9%, respectively, compared with fermentations in which DO concentrations were kept constant at 80%, 60%, and 30%.
Yong-Qian Fu; Shuang Li; Yao Chen; Qing Xu; He Huang; Xiao-Yan Sheng
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-21
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  162     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-13     Completed Date:  2010-12-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1031-8     Citation Subset:  IM    
College of Biotechnology and Pharmaceutical Engineering, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China.
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MeSH Terms
Bioreactors / microbiology
Culture Techniques / methods*
Fumarates / metabolism*
Glucose / metabolism
Oxygen / metabolism*
Rhizopus / metabolism*
Reg. No./Substance:
0/Fumarates; 110-17-8/fumaric acid; 50-99-7/Glucose; 7782-44-7/Oxygen

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