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Enhancement of DNA vaccine efficacy by targeting the xenogeneic hCG, survivin and VEGFR 2 combined tumor antigen to the MHC II pathway.
MedLine Citation:
PMID:  22438278     Owner:  NLM     Status:  Publisher    
BACKGROUND: Quite a number of strategies have been used to improve the efficacy of the DNA vaccine for the treatment of tumors. These strategies, ranging from activating CD4(+) T cell, manipulating antigen presentation, and/or processing to anti-angiogenesis, focus on one certain aspect in the functioning of the vaccine. Therefore, combination is necessary for rational DNA vaccines design by synergizing different regimens and overcoming the limitations of each strategy. METHODS: A DNA fragment (HSV) encoding the C terminal 37aa of human chorionic gonadotropin β chain (hCGβ), 5 different HLA-restricted cytotoxic T lymphocyte (CTL) epitopes from human survivin and the 3rd and 4th extracellular domains of vascular endothelial growth factor receptor 2 (VEGFR2) was inserted into the sequence between the luminal and transmembrane domain of human lysosome-associated membrane protein-1 (LAMP-1) cDNA for the construction of a novel DNA vaccine. RESULTS: This novel vaccine, named p-L/HSV, has a potent antitumor effect on the LL/2 lung carcinoma model in syngeneic C57BL/6 mice. The immunologic mechanism involved in the antitumor effect referred to the activation of both cellular and humoral immune response. In addition, the tumor vasculature was abrogated as observed by immunohistochemistry in p-L/HSV immunized mice. Furthermore, the mice received an additional boost with p-L/HSV 6 months later showed a strong immune recall response. CONCLUSIONS: Our study indicates that the strategies of combining antitumor with antiangiogenesis and targeting the tumor antigen to the MHC II pathway cooperate well. Such a study may shed new light on designing vaccine for cancer in the future. Copyright © 2012 John Wiley & Sons, Ltd.
Yuying Wei; Yuanjie Sun; Chaojun Song; Haitao Li; Yongming Li; Kui Zhang; Jiuyu Gong; Fei Liu; Zhijia Liu; Jt August; Boquan Jin; Kun Yang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-21
Journal Detail:
Title:  The journal of gene medicine     Volume:  -     ISSN:  1521-2254     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815764     Medline TA:  J Gene Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Department of Immunology, The Fourth Military Medical University, No.169 Changle West Road, Xi'an, 710032, Shaanxi Province, P. R. of China.
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