| Enhancement of DNA micro-array analysis using a shear-driven micro-channel flow system. | |
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MedLine Citation:
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PMID: 14558606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A very simple micro-channel flow system is used to investigate the potential gain in hybridization rate stemming from the induction of a convective flow past the surface of a DNA micro-array. Reporting on a series of preliminary experiments wherein a two-dimensional convective flow is created past the surface of a conventional micro-array slide, the analysis time could be brought down from overnight waiting (16 h) to some 10 to 30 min. The experiments open the road towards the development of novel, convection-driven hybridization systems yielding shorter analysis times, and/or lower detection limits. |
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Authors:
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K Pappaert; J Vanderhoeven; P Van Hummelen; B Dutta; D Clicq; G V Baron; G Desmet |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 1014 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2003 Oct |
Date Detail:
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Created Date: 2003-10-15 Completed Date: 2004-05-11 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: Netherlands |
Other Details:
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Languages: eng Pagination: 1-9 Citation Subset: IM |
Affiliation:
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Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, 1050 Brussels, Belgium. kpappaer@vub.ac.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Oligonucleotide Array Sequence Analysis* Sensitivity and Specificity |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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