Document Detail


Enhancement of adipose-derived stem cell differentiation in scaffolds with IGF-I gene impregnation under dynamic microenvironment.
MedLine Citation:
PMID:  20408758     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Biochemical and mechanical signals enabling cardiac regeneration can be elucidated by using in vitro tissue engineering models. We hypothesized that human insulin-like growth factor-I (IGF-I) and 3-dimensional (3D) dynamic microenvironment could enhance the survival and differentiation of adipose tissue-derived stem cells (ADSCs). In this study, ADSCs were cultured on 3D porous scaffolds with or without plasmid DNA PIRES2-IGF-I in cardiac media, in static culture dishes, and in a spinning flask bioreactor, respectively. Cell viability, formation of cardiac-like structure, expression of functional proteins, and gene expressions were tested in the cultured constructs on day 14. The results showed that dynamic microenvironment enhanced the release of plasmid DNA; the ADSCs can be transfected by the released plasmid DNA PIRES2-IGF-I in scaffold. IGF-I showed beneficial effects on cellular viability and increase of total protein and also increased the expressions of cardiac-specific proteins and genes in the grafts. It was also demonstrated that dynamic stirring environment could promote the proliferation of ADSCs. Therefore, IGF-I, expressed by ADSCs transfected by DNA PIRES2-IGF-I incorporated into scaffold, and hydrodynamic microenvironment can independently and interactively increase cellular viability and interactively increase the expression of cardiac-specific proteins and genes in the grafts. The results would be useful for developing tissue-engineered grafts for myocardial repair.
Authors:
Yanxia Zhu; Tianqing Liu; Hua Ye; Kedong Song; Xuehu Ma; Zhanfeng Cui
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Stem cells and development     Volume:  19     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2011-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1547-56     Citation Subset:  IM    
Affiliation:
Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, China.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / cytology*
Biological Markers / metabolism
Cell Differentiation / physiology*
Humans
Insulin-Like Growth Factor I / genetics*,  metabolism
Plasmids / genetics,  metabolism
Stem Cells / cytology,  physiology*
Tissue Engineering / methods*
Tissue Scaffolds*
Chemical
Reg. No./Substance:
0/Biological Markers; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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