| Enhancement of the ALP activity of C3H10T1/2 cells by the combination of an oxysterol and apatite. | |
| | |
MedLine Citation:
|
PMID: 20683129 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Biomimetic apatite coating has been used to load osteogenic biomolecules onto the surface of titanium implants. Apatite on the surface of biomaterials is thought to function as a reservoir of biomolecules as well as enhancing osteoconductivity. In this study, 20alpha-hydroxycholesterol (20alpha-HC), an osteogenic oxysterol, was used to induce differentiation of a mouse embryo fibroblast cell line (C3H10T1/2) by loading the oxysterol on biomimetically coated apatite of titanium discs. We found that the phosphatase (alkaline phosphatase (ALP)) activity of 20alpha-HC was significantly higher with ascorbic acid than alone, suggesting a need for ascorbic acid as a co-factor. When 20alpha-HC was added into the apatite coating solution, the ALP activity of the C3H10T1/2 cells did not increase on the apatite surface, even in the presence of ascorbic acid. However, ALP activity increased dramatically when 20alpha-HC was loaded by volatilization of EtOH from the apatite coat after dipping discs in 20alpha-HC-dissolved EtOH. Interestingly, ascorbic acid was not needed for this increase in ALP activity, suggesting a synergistic effect of 20alpha-HC and apatite. The concentration of calcium ions, a major component of apatite, affected the osteogenic effect of 20alpha-HC, and the increase in ALP activity was attenuated by L-type calcium channel inhibitors, verapamil and nifedipine. These results demonstrate that calcium ions released from apatite are important in the synergistic effect of 20alpha-HC and apatite. |
| | |
Authors:
|
Kyung Mi Son; Hee Chul Park; Na Ryoung Kim; In-Seop Lee; Hyeong-Cheol Yang |
Related Documents
:
|
196059 - Phagocytosis and leukocyte enzymes in ascorbic acid deficient guinea pigs. 9680239 - Kainic acid causes redox changes in cerebral cortex extracellular fluid: nmda receptor ... 1650329 - Opisthorchis viverrini infestation and endogenous nitrosamines as risk factors for chol... 15590689 - 6-bromo-6-deoxy-l-ascorbic acid: an ascorbate analog specific for na+-dependent vitamin... 2674049 - The effect of ascorbate on proteolytic enzyme activities in fish. 15745839 - Ascorbic acid and uric acid suppress glucose-induced fibronectin and vascular endotheli... 10653329 - Erythrocyte fatty acid composition in term infants fed human milk or a formula enriched... 11975529 - Diastereoselective synthesis and estimation of the conformational flexibility of 6-oxop... 1619189 - Changes in serum and urinary uric acid levels in normal human subjects fed purine-rich ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-03 |
Journal Detail:
|
Title: Biomedical materials (Bristol, England) Volume: 5 ISSN: 1748-605X ISO Abbreviation: Biomed Mater Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-08-04 Completed Date: 2011-01-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101285195 Medline TA: Biomed Mater Country: England |
Other Details:
|
Languages: eng Pagination: 044107 Citation Subset: IM |
Affiliation:
|
Department of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National University, Yeonkun-dong, Chongro-ku, Seoul 110-749, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkaline Phosphatase
/
metabolism* Animals Apatites / chemistry* Cell Culture Techniques / methods Cell Line Cell Proliferation Cell Survival Coated Materials, Biocompatible / chemistry, pharmacology* Enzyme Activation / drug effects Fibroblasts / cytology, drug effects, physiology* Hydroxycholesterols / chemistry, pharmacology* Materials Testing Mice Tissue Engineering / methods* Titanium / chemistry* |
| Chemical | |
Reg. No./Substance:
|
0/Apatites; 0/Coated Materials, Biocompatible; 0/Hydroxycholesterols; 516-72-3/20-hydroxycholesterol; 7440-32-6/Titanium; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Study of synthesis of nano-hydroxyapatite using a silk fibroin template.
Next Document: Wear performance of self-mating contact pairs of TiN and TiAlN coatings on orthopedic grade Ti-6Al-4...