| Enhancement of 5-Aminolevulinic acid-induced oxidative stress on two cancer cell lines by gold nanoparticles. | |
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MedLine Citation:
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PMID: 19905984 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5-Aminolevulinic acid (5-ALA) and its methyl ester (5-ALA-Me) at mM concentration levels induce oxidative stress via the production of reactive oxygen species (ROS). Human cancer cell lines (MCF-7 and HepG2) incubated in the dark in the simultaneous presence of 5.0 mM or more 5-ALA or 5-ALA-Me (for MCF-7) and 7 microg/mL of 15 nm citrate capped gold nanoparticles (AuNPs) were damaged more seriously compared to those in the presence of the levulinic acid alone. Damage is visible in electron micrographs which reveal similar morphology both in the presence or absence of AuNPs. Cytotoxicity was observed irrespective of the presence of serum and medium. Production of ROS in cell free samples containing 5-ALA-Me was monitored by EPR as the DMPO-OH spin adduct and also showed a catalytic effect of AuNPs. Both SOD and CAT inhibited the production of ROS and also reduced cytotoxicity in the cell samples. These observations can be explained by initial attack on the cell membrane by ROS produced in the medium outside the cell and provide insight into possible uses of 5-ALA in cancer chemotherapy. |
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Authors:
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Shinji Ito; Norio Miyoshi; William G Degraff; Kunio Nagashima; Louis J Kirschenbaum; Peter Riesz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Free radical research Volume: 43 ISSN: 1029-2470 ISO Abbreviation: Free Radic. Res. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-11-12 Completed Date: 2010-02-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 1214-24 Citation Subset: IM |
Affiliation:
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Radiation Biology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-1002, USA. itoshi@mail.nih.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminolevulinic Acid
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pharmacology* Cell Line, Tumor Cell Survival / drug effects Gold / pharmacology* Humans Metal Nanoparticles* / ultrastructure Microscopy, Electron, Transmission Neoplasms / metabolism*, pathology, ultrastructure Oxidative Stress / drug effects* Reactive Oxygen Species / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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N0I-CO-12400/CO/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 106-60-5/Aminolevulinic Acid; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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