| Enhancement of BALB/c 3T3 cells transformation by 1,2-dibromoethane promoting effect. | |
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MedLine Citation:
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PMID: 8625443 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two of the most representative halogenated aliphatic hydrocarbons, 1,2-dibromoethane and 1,1,2,2-tetrachloroethane, were tested in the two-stage cell transformation model for analysing the promoting ability. Both of these compounds had previously been found to exert genotoxic effects, probably acting as moderate initiators. BALB/c 3T3 cells were initiated with subtransforming doses of N-methyl-N-nitro-N-nitrosoguanidine or 3-methylcholanthrene and then exposed to a chronic treatment with different non-transforming dosages of the two haloalkanes. 1,1,2,2-Tetrachloroethane did not exert any promoting activity in that system. By contrast, significant promoting effects by 1,2-dibromoethane were observed both in cells treated with N-methyl-N-nitro-N-nitrosoguanidine and in cells treated with 3-methylcholanthrene. Promotion of the transformation process initiated with 3-methylcholanthrene was detectable when confluent cells in the chemical-treated plates were replated in the level-II amplification test. This experimental procedure allowed cells to perform further rounds of replications and transformed foci to became detectable. Results gave evidence for a promoting role of 1,2-dibromoethane in multistep carcinogenesis, probably responsible for the higher oncogenic ability of this compound with respect to 1,1,2,2-tetrachloroethane. |
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Authors:
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A Colacci; M Vaccari; P Perocco; C Da Vià; P Silingardi; E Manzini; W Horn; S Grilli |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carcinogenesis Volume: 17 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1996 Feb |
Date Detail:
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Created Date: 1996-06-24 Completed Date: 1996-06-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 225-31 Citation Subset: IM |
Affiliation:
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Istituto Nazionale per la Ricerca sul Cancro (IST)-Genova, Biotechnology Satellite Unit Bologna, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells
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drug effects*,
pathology Animals Carcinogens / toxicity* Cell Transformation, Neoplastic / chemically induced*, pathology Dose-Response Relationship, Drug Ethane / analogs & derivatives*, toxicity Ethylene Dibromide / toxicity* Hydrocarbons, Chlorinated / toxicity* Methylnitronitrosoguanidine Mice Tetradecanoylphorbol Acetate |
| Chemical | |
Reg. No./Substance:
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0/Carcinogens; 0/Hydrocarbons, Chlorinated; 106-93-4/Ethylene Dibromide; 16561-29-8/Tetradecanoylphorbol Acetate; 70-25-7/Methylnitronitrosoguanidine; 74-84-0/Ethane; 79-34-5/1,1,2,2-tetrachloroethane |
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