Document Detail

Enhancement of BALB/c 3T3 cells transformation by 1,2-dibromoethane promoting effect.
MedLine Citation:
PMID:  8625443     Owner:  NLM     Status:  MEDLINE    
Two of the most representative halogenated aliphatic hydrocarbons, 1,2-dibromoethane and 1,1,2,2-tetrachloroethane, were tested in the two-stage cell transformation model for analysing the promoting ability. Both of these compounds had previously been found to exert genotoxic effects, probably acting as moderate initiators. BALB/c 3T3 cells were initiated with subtransforming doses of N-methyl-N-nitro-N-nitrosoguanidine or 3-methylcholanthrene and then exposed to a chronic treatment with different non-transforming dosages of the two haloalkanes. 1,1,2,2-Tetrachloroethane did not exert any promoting activity in that system. By contrast, significant promoting effects by 1,2-dibromoethane were observed both in cells treated with N-methyl-N-nitro-N-nitrosoguanidine and in cells treated with 3-methylcholanthrene. Promotion of the transformation process initiated with 3-methylcholanthrene was detectable when confluent cells in the chemical-treated plates were replated in the level-II amplification test. This experimental procedure allowed cells to perform further rounds of replications and transformed foci to became detectable. Results gave evidence for a promoting role of 1,2-dibromoethane in multistep carcinogenesis, probably responsible for the higher oncogenic ability of this compound with respect to 1,1,2,2-tetrachloroethane.
A Colacci; M Vaccari; P Perocco; C Da Vià; P Silingardi; E Manzini; W Horn; S Grilli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carcinogenesis     Volume:  17     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1996 Feb 
Date Detail:
Created Date:  1996-06-24     Completed Date:  1996-06-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  225-31     Citation Subset:  IM    
Istituto Nazionale per la Ricerca sul Cancro (IST)-Genova, Biotechnology Satellite Unit Bologna, Italy.
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MeSH Terms
3T3 Cells / drug effects*,  pathology
Carcinogens / toxicity*
Cell Transformation, Neoplastic / chemically induced*,  pathology
Dose-Response Relationship, Drug
Ethane / analogs & derivatives*,  toxicity
Ethylene Dibromide / toxicity*
Hydrocarbons, Chlorinated / toxicity*
Tetradecanoylphorbol Acetate
Reg. No./Substance:
0/Carcinogens; 0/Hydrocarbons, Chlorinated; 106-93-4/Ethylene Dibromide; 16561-29-8/Tetradecanoylphorbol Acetate; 70-25-7/Methylnitronitrosoguanidine; 74-84-0/Ethane; 79-34-5/1,1,2,2-tetrachloroethane

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