| Enhanced welan gum production using a two-stage agitation speed control strategy in Alcaligenes sp. CGMCC2428. | |
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MedLine Citation:
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PMID: 20640447 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Batch fermentative production of welan gum by Alcaligenes sp. CGMCC2428 was investigated under various oxygen supply conditions using regulating agitation speed. Based on a three kinetic parameters analysis that includes specific cell growth rate (μ), specific glucose consumption rate (q (s)), and specific welan formation rate (q (p)), a two-stage agitation speed control strategy was proposed to achieve high concentration, high yield, and high viscosity of welan. During the first 22 h, the agitation speed in 7.5 L fermenter was controlled at 800 rpm to maintain high μ for cell growth. The agitation was then reduced step-wise to 600 rpm to maintain a changing profile with stable dissolved oxygen levels and obtain high qp for high welan accumulation. Finally, the maximum concentration of welan was reached at 26.3 ± 0.89 g L(-1) with a yield of 0.53 ± 0.003 g g(-1) and the welan gum viscosity of 3.05 ± 0.10 Pa s, which increased by an average of 15.4, 15.2, and 20.1% over the best results controlled by constant agitation speeds. |
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Authors:
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Hui Li; Hong Xu; Hao Xu; Sha Li; Han-Jie Ying; Ping-Kai Ouyang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-17 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: 34 ISSN: 1615-7605 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: Germany |
Other Details:
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Languages: eng Pagination: 95-102 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 5 Xinmofan Road, Gulou District, Nanjing 210009, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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