Document Detail


Enhanced visualization of axonally transported proteins in the immature CNS by suppression of systemic labeling.
MedLine Citation:
PMID:  2435374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the neonate hamster, visualization of axonally transported proteins in the retinofugal pathway is obscured by high levels of systemic (background) labeling. Radiolabeled precursors injected into the eye diffuse rapidly into the general circulation and then across the immature blood-brain barrier to be incorporated into proteins that are synthesized throughout the brain. Systemic labeling can be suppressed, however, by i.p. injections of large amounts of either non-radioactive methionine 30 min after intraocular labeling with [35S]methionine, or non-radioactive leucine given at the time of intraocular labeling. Whereas the former competes with the radioactive precursor during incorporation into brain proteins (after most of the retinal labeling has already been achieved), the latter competes at the earlier stage of access to the brain. Both methods reduced background labeling by more than 60%, thereby allowing for unambiguous identification of axonally transported proteins. The pattern of rapidly transported proteins was found to be strikingly different between neonates and mature animals, including marked changes in an identified 'growth-associated protein' (50 kDa, pI 4.8).
Authors:
K L Moya; L I Benowitz; S Jhaveri; G E Schneider
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  428     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-05-15     Completed Date:  1987-05-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  183-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Animals
Animals, Newborn / metabolism*
Axonal Transport
Binding, Competitive
Central Nervous System / metabolism*
Cricetinae
Eye
Image Enhancement / methods*
Injections
Mesocricetus
Methionine / pharmacology
Nerve Tissue Proteins / metabolism*
Superior Colliculi / metabolism
Grant Support
ID/Acronym/Agency:
EY00126/EY/NEI NIH HHS; EY02621/EY/NEI NIH HHS; EY05504/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Nerve Tissue Proteins; 63-68-3/Methionine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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