Document Detail


Enhanced tissue production through redox control in stem cell-laden hydrogels.
MedLine Citation:
PMID:  23627869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular bioenergetics and redox (reduction-oxidation) play an important role in cell proliferation and differentiation, key aspects of building new tissues. In the present study, we examined the metabolic characteristics of human adipose-derived stem cells (hASCs) during proliferation and differentiation in both monolayer and three-dimensional biomaterial scaffolds. In monolayer, hASCs exhibited higher glycolysis and lower ox-phos as compared to both adipogenic and osteogenic differentiated cells, and hASCs demonstrated the Warburg effect (aerobic glycolysis). However, reactive oxygen species (ROS) levels increased during adipogenic differentiation, but decreased during osteogenic differentiation. Similarly, a decrease in ROS levels along with a higher mitochondrial membrane potential and viability was observed in hASCs encapsulated in poly(ethylene glycol) (PEG) hydrogels containing an adhesion peptide (RGD), compared to PEG hydrogels with a scrambled control peptide (GRD), demonstrating that adhesion-dependent signaling can also regulate ROS production and bioenergetics. As a result, we hypothesized that we could modulate osteogenesis in PEG hydrogels containing the adhesion peptide (RGD) by further reducing ROS levels using a small therapeutic molecule, L-carnitine, a metabolite with purported antioxidant effects. We observed reduced ROS levels, no effect on mitochondrial membrane potential, and increased osteogenic differentiation and tissue production in cells in the presence of L-carnitine. These results suggest the potential to manipulate tissue production by modulating cellular metabolism.
Authors:
Branden Reid; Junaid M Afzal; Annemarie M McCartney; M Roselle Abraham; Brian O'Rourke; Jennifer H Elisseeff
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-06-22
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  19     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-07-29     Completed Date:  2014-03-13     Revised Date:  2014-09-02    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2014-23     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipogenesis / physiology
Adipose Tissue / cytology
Cell Differentiation / physiology
Cells, Cultured
Humans
Hydrogels / chemistry*
Osteogenesis / physiology
Oxidation-Reduction
Polyethylene Glycols / chemistry
Stem Cells / cytology*
Tissue Engineering / methods
Grant Support
ID/Acronym/Agency:
3R01DE016887-03S/DE/NIDCR NIH HHS; R01 HL105216/HL/NHLBI NIH HHS; R01AR054005/AR/NIAMS NIH HHS; T32 GM007309/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Hydrogels; 0/Polyethylene Glycols
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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