Document Detail

Enhanced synthesis of L-threo-3,4-dihydroxyphenylserine by high-density whole-cell biocatalyst of recombinant L-threonine aldolase from Streptomyces avelmitilis.
MedLine Citation:
PMID:  19039530     Owner:  NLM     Status:  MEDLINE    
L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10 degrees C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions.
Sang-Ho Baik; Hideki Yoshioka
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-28
Journal Detail:
Title:  Biotechnology letters     Volume:  31     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-02-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  443-8     Citation Subset:  IM    
Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju, Jeonbuk, 561-756, South Korea.
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MeSH Terms
Antiparkinson Agents / metabolism*
Benzaldehydes / metabolism
Biotechnology / methods*
Catechols / metabolism
Droxidopa / metabolism*
Escherichia coli / genetics,  metabolism*
Glycine / metabolism
Glycine Hydroxymethyltransferase / genetics,  metabolism*
Streptomyces / enzymology*,  genetics
Threonine / metabolism*
Reg. No./Substance:
0/Antiparkinson Agents; 0/Benzaldehydes; 0/Catechols; 139-85-5/protocatechualdehyde; 23651-95-8/Droxidopa; 56-40-6/Glycine; 72-19-5/Threonine; EC Hydroxymethyltransferase

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