| Enhanced synthesis of L-threo-3,4-dihydroxyphenylserine by high-density whole-cell biocatalyst of recombinant L-threonine aldolase from Streptomyces avelmitilis. | |
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MedLine Citation:
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PMID: 19039530 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned from Streptomyces avelmitilis MA-4680. Maximum conversion rates were observed at 2 M glycine, 145 mM 3,4-dihydroxybenzaldehyde, 0.75% Triton-X, 5 g E. coli cells/l, pH 6.5 and 10 degrees C. In the optimized condition, overall productivity was 8 g/l, which represents 40 times the synthesis yield possible with no optimization of conditions. |
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Authors:
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Sang-Ho Baik; Hideki Yoshioka |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-28 |
Journal Detail:
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Title: Biotechnology letters Volume: 31 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-02 Completed Date: 2009-02-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 443-8 Citation Subset: IM |
Affiliation:
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Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju, Jeonbuk, 561-756, South Korea. baiksh@chonbuk.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antiparkinson Agents
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metabolism* Benzaldehydes / metabolism Biotechnology / methods* Catechols / metabolism Droxidopa / metabolism* Escherichia coli / genetics, metabolism* Glycine / metabolism Glycine Hydroxymethyltransferase / genetics, metabolism* Streptomyces / enzymology*, genetics Threonine / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Antiparkinson Agents; 0/Benzaldehydes; 0/Catechols; 139-85-5/protocatechualdehyde; 23651-95-8/Droxidopa; 56-40-6/Glycine; 72-19-5/Threonine; EC 2.1.2.1/Glycine Hydroxymethyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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