Document Detail


Enhanced stimulation of chromosomal translocations by radiomimetic DNA damaging agents and camptothecin in Saccharomyces cerevisiae rad9 checkpoint mutants.
MedLine Citation:
PMID:  15013706     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Saccharomyces cerevisiae rad9 checkpoint mutants exhibit pleiotropic phenotypes, including higher frequencies of chromosome loss, radiation sensitivity, and decreased induction of DNA damage-inducible genes. We had previously shown that rad9 mutants exhibit higher frequencies of DNA damage-associated translocations but lower frequencies of DNA damage-associated sister chromatid exchange (SCE), compared to wild type. Herein, we have shown that differences between the frequencies of DNA damage-associated recombination in the rad9 mutant and wild type depend on the identity and the concentration of the DNA damaging agent. Translocation and SCE frequencies were measured in strains containing truncated his3 fragments, located either on chromosomes II and IV, or located in tandem on chromosome IV, respectively. DNA damage-associated frequencies of translocations after exposure to hydrogen peroxide (H(2)O(2)), bleomycin, phleomycin, cisplatin, and camptothecin are higher in the rad9 diploid than in wild type. However, translocation frequencies after exposure to 4-nitroquinoline 1-oxide (4-NQO) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are similar in rad9 and wild-type strains. We suggest that the deficiency in triggering G(2) arrest after exposure to specific DNA damaging agents results in the higher levels of DNA damage-associated translocations in rad9 mutants.
Authors:
Michael Fasullo; Li Zeng; Peter Giallanza
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Mutation research     Volume:  547     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-03-11     Completed Date:  2004-05-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  123-32     Citation Subset:  IM    
Affiliation:
Ordway Research Institute, The Albany Medical College, 150 New Scotland Ave., Albany, NY 12208, USA. mfasullo@ordwayresearch.org
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MeSH Terms
Descriptor/Qualifier:
4-Nitroquinoline-1-oxide / toxicity
Camptothecin / toxicity*
Cell Cycle Proteins / genetics*
Chromosomes, Fungal / drug effects*
DNA Damage*
DNA, Fungal / radiation effects*
Dose-Response Relationship, Drug
Enzyme Inhibitors / toxicity
Methylnitronitrosoguanidine / toxicity
Mutagens / toxicity*
Mutation*
Recombination, Genetic / radiation effects
Saccharomyces cerevisiae / genetics,  radiation effects*
Saccharomyces cerevisiae Proteins / genetics*
Translocation, Genetic
Grant Support
ID/Acronym/Agency:
CA70105/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/DNA, Fungal; 0/Enzyme Inhibitors; 0/Mutagens; 0/Saccharomyces cerevisiae Proteins; 139691-42-2/rad9 protein; 56-57-5/4-Nitroquinoline-1-oxide; 70-25-7/Methylnitronitrosoguanidine; 7689-03-4/Camptothecin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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