Document Detail


Enhanced stability and therapeutic utility of proteins upon conjugation with hydrophilic polymers.
MedLine Citation:
PMID:  8181948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Pharmacological utility of various exogenous proteins is considerably impeded, since upon infusion, they are liable to rapid systemic clearance, proteolysis, in addition to eliciting severe immunological reactions. Upon conjugation with hydrophilic polymers like polyethylene glycol and dextran, the altered topology of proteins, often hinders interaction with their corresponding complementary surfaces. This is manifest in their increased circulatory-lives, as a fall out of decreased binding to proteases, pre-existing antibodies and other opsonins that mediate clearance by the reticuloendothelial-system as well as receptors mediating specific organ uptake. Further, an increase in conjugate size beyond the threshold limit of 70 kDa, permits small proteins to escape clearance via renal filtration. Upon covalent modification, proteins are generally endowed with an intrinsic inertness which is reflected in their enhanced stability in extremes of pH and temperature, presence of proteases and other denaturing conditions. The enhanced intracellular stability of conjugates which may also be responsible for their altered immunological properties is likely to be a consequence of the changed physiochemical properties of the conjugates. The therapeutic efficacy of these conjugates in clinical trials indicate their tremendous utility in pharmacological procedures.
Authors:
G Mitra; S Mumtaz; B K Bachhawat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Hindustan antibiotics bulletin     Volume:  35     ISSN:  0018-1935     ISO Abbreviation:  Hindustan Antibiot Bull     Publication Date:    1993 Feb-May
Date Detail:
Created Date:  1994-06-13     Completed Date:  1994-06-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985097R     Medline TA:  Hindustan Antibiot Bull     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  133-56     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Delhi South Campus, India.
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MeSH Terms
Descriptor/Qualifier:
Chemistry, Pharmaceutical / methods*
Drug Design
Drug Stability
Polymers / chemistry*,  therapeutic use
Proteins / chemistry*,  therapeutic use
Chemical
Reg. No./Substance:
0/Polymers; 0/Proteins

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